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Immunohistochemistry (IHC) (Anti-NELFA / WHSC2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Mouse anti-Human, Rat NELFA / WHSC2 Monoclonal Antibody | anti-NELFA antibody

Mouse Monoclonal [clone 6B11H8] (IgG2b) to Human NELFA / WHSC2

Gene Names
NELFA; WHSC2; NELF-A; P/OKcl.15
Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Purified
Synonyms
NELFA / WHSC2; Monoclonal Antibody; Mouse Monoclonal [clone 6B11H8] (IgG2b) to Human NELFA / WHSC2; Anti-NELFA / WHSC2 Antibody (aa280-511; clone 6B11H8) IHC-plus; NELFA; Negative elongation factor A; NELF-A; P/OKcl.15; WHSC2; Human NELFA; anti-NELFA antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
6B11H8
Specificity
Human WHSC2
Purity/Purification
Purified
Form/Format
PBS, 0.05% sodium azide, 0.5% protein stabilizer
Concentration
1 mg/ml (varies by lot)
Sequence Length
539
Applicable Applications for anti-NELFA antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
ELISA (1:10000), Flo (1:200 - 1:400), ICC (1:200 - 1:1000), IHC-P (10 ug/ml), WB (1:500 - 1:2000)
Target Species
Human
Immunogen Description
Purified recombinant fragment of human WHSC2 (AA: 280-511)
Immunogen Type
Recombinant protein
Antigen Modification
aa280-511
Preparation and Storage
Short term +4 degree C; Long term -20 degree C

Immunohistochemistry (IHC)

(Anti-NELFA / WHSC2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC) (Anti-NELFA / WHSC2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Flow Cytometry (FC/FACS)

(Flow cytometry of HEK293 cells using WHSC2 mouse mAb (green) and negative control (purple).)

Flow Cytometry (FC/FACS) (Flow cytometry of HEK293 cells using WHSC2 mouse mAb (green) and negative control (purple).)

Western Blot (WB)

(Western blot of WHSC2 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A549 (5), SPC-A-1 (6) cell lysate, and Rat brain (4) tissue lysate.)

Western Blot (WB) (Western blot of WHSC2 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A549 (5), SPC-A-1 (6) cell lysate, and Rat brain (4) tissue lysate.)

ELISA (EIA)

(Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)

ELISA (EIA) (Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,416 Da
NCBI Official Full Name
negative elongation factor A
NCBI Official Synonym Full Names
negative elongation factor complex member A
NCBI Official Symbol
NELFA
NCBI Official Synonym Symbols
WHSC2; NELF-A; P/OKcl.15
NCBI Protein Information
negative elongation factor A; wolf-Hirschhorn syndrome candidate 2 protein
UniProt Protein Name
Negative elongation factor A
UniProt Gene Name
NELFA
UniProt Synonym Gene Names
WHSC2; NELF-A
UniProt Entry Name
NELFA_HUMAN

NCBI Description

This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008]

Uniprot Description

WHSC2: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex. Belongs to the NELF-A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; multicellular organismal development; RNA elongation from RNA polymerase II promoter; gene expression

Research Articles on NELFA

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Product Notes

The NELFA nelfa (Catalog #AAA249187) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 6B11H8] (IgG2b) to Human NELFA / WHSC2 reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NELFA / WHSC2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA). ELISA (1:10000), Flo (1:200 - 1:400), ICC (1:200 - 1:1000), IHC-P (10 ug/ml), WB (1:500 - 1:2000). Researchers should empirically determine the suitability of the NELFA nelfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NELFA / WHSC2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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