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Immunohistochemistry (IHC) (Anti-CFL2 / Cofilin 2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Goat CFL2 / Cofilin 2 Polyclonal Antibody | anti-CFL2 antibody

Goat Polyclonal to Human CFL2 / Cofilin 2

Gene Names
CFL2; NEM7
Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rat
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Immunoaffinity Purified
Synonyms
CFL2 / Cofilin 2; Polyclonal Antibody; Goat Polyclonal to Human CFL2 / Cofilin 2; Anti-CFL2 / Cofilin 2 Antibody (C-Terminus) IHC-plus; CFL2; Cofilin 2; Cofilin-2; NEM7; Cofilin 2 (muscle); Cofilin; muscle isoform; Human CFL2; anti-CFL2 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rat
Clonality
Polyclonal
Specificity
Human CFL2. This antibody is expected to recognise both reported isoforms (NP_068733 and NP_619579)
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
166
Applicable Applications for anti-CFL2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA (1:128000), IHC-P (3.75 ug/ml), WB (0.01 - 0.03 ug/ml)
Target Species
Human
Immunogen Description
Synthetic peptide C-DIKDRSTLGEK from the C-terminus of human CFL2 (NP_068733.1; NP_619579.1).
Immunogen Type
Synthetic peptide
Immunogen
CFL2 / Cofilin 2 antibody was raised against synthetic peptide C-DIKDRSTLGEK from the C-terminus of human CFL2 (NP_068733.1; NP_619579.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus (100%); Baboon, Lizard (91%).
Antigen Modification
C-Terminus
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CFL2 / Cofilin 2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-CFL2 / Cofilin 2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

((0.003 ug/ml) Staining of Human Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.003 ug/ml) Staining of Human Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Product Categories/Family for anti-CFL2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
– Da
NCBI Official Full Name
cofilin-2 isoform 1
NCBI Official Synonym Full Names
cofilin 2 (muscle)
NCBI Official Symbol
CFL2
NCBI Official Synonym Symbols
NEM7
NCBI Protein Information
cofilin-2; cofilin, muscle isoform; nemaline myopathy type 7
UniProt Protein Name
Cofilin-2
Protein Family
UniProt Gene Name
CFL2
UniProt Entry Name
COF2_HUMAN

NCBI Description

This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]

Uniprot Description

Cofilin-2: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Nuclear receptor co-regulator; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: I band; extracellular space; nuclear matrix; actin cytoskeleton

Molecular Function: protein binding; actin binding

Biological Process: muscle maintenance; actin filament depolymerization; positive regulation of actin filament depolymerization; sarcomere organization

Disease: Nemaline Myopathy 7

Research Articles on CFL2

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Product Notes

The CFL2 cfl2 (Catalog #AAA248735) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human CFL2 / Cofilin 2 reacts with Bat, Gibbon, Bovine, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CFL2 / Cofilin 2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA (1:128000), IHC-P (3.75 ug/ml), WB (0.01 - 0.03 ug/ml). Researchers should empirically determine the suitability of the CFL2 cfl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CFL2 / Cofilin 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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