Rabbit anti-Human SCUBE3 Polyclonal Antibody | anti-SCUBE3 antibody

Rabbit Polyclonal to Human SCUBE3

Cat. Num. AAA248351

• Gene Names: SCUBE3; CEGF3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: SCUBE3; Polyclonal Antibody; Rabbit Polyclonal to Human SCUBE3; Anti-SCUBE3 Antibody (Internal) IHC-plus; CEGF3; Human SCUBE3; anti-SCUBE3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human SCUBE3. At least two isoforms of SCUBE3 are known to exist. SCUBE3 antibody is predicted to not cross-react with other SCUBE members.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 993

• Applicable Applications for anti-SCUBE3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (1 - 2 ug/ml); Usage: SCUBE3 antibody can be used for detection of SCUBE3 by Western blot at 1-2 ug/mL.
• Target Species: Human
• Immunogen Description: A 15 amino acid peptide near the center of human SCUBE3.
• Immunogen Type: Synthetic peptide
• Immunogen: SCUBE3 antibody was raised against a 15 amino acid peptide near the center of human SCUBE3.
• Antigen Modification: Internal
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-SCUBE3 antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Immunofluorescence of SCUBE3 in human kidney tissue with SCUBE3 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot analysis of SCUBE3 in mouse kidney tissue lysate with SCUBE3 antibody at (A) 1 and (B) 2 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 31377568
• NCBI GeneID: 222663
• NCBI Accession #: NP_689966.2
• NCBI GenBank Nucleotide #: NM_152753.3
• UniProt Accession #: Q8IX30; Q5CZB3; Q86UZ9; Q8NAU9; A8K5A3
• Molecular Weight: 111,099 Da
• NCBI Official Full Name: signal peptide, CUB and EGF-like domain-containing protein 3 isoform 1
• NCBI Official Synonym Full Names: signal peptide, CUB domain, EGF-like 3
• NCBI Official Symbol: SCUBE3
• NCBI Official Synonym Symbols: CEGF3
• NCBI Protein Information: signal peptide, CUB and EGF-like domain-containing protein 3; CUB domain and EGF-like repeat containing 3; signal peptide, CUB and EGF-like domain containing protein 3; signal peptide-, CUB domain-, and EGF-like domains-containing protein 3
• UniProt Protein Name: Signal peptide, CUB and EGF-like domain-containing protein 3
• Protein Family: Signal peptide, CUB and EGF-like domain-containing protein
• UniProt Gene Name: SCUBE3
• UniProt Entry Name: SCUB3_HUMAN

NCBI Description

This gene encodes a member of the signal peptide, complement subcomponents C1r/C1s, Uegf, bone morphogenetic protein-1 and epidermal growth factor-like domain containing protein family. Overexpression of this gene in human embryonic kidney cells results in secretion of a glycosylated form of the protein that forms oligomers and tethers to the cell surface. This gene is upregulated in lung cancer tumor tissue compared to healthy tissue and is associated with loss of the epithelial marker E-cadherin and with increased expression of vimentin, a mesenchymal marker. In addition, the protein encoded by this gene is a transforming growth factor beta receptor ligand, and when secreted by cancer cells, it can be cleaved in vitro to release the N-terminal epidermal growth factor-like repeat domain and the C-terminal complement subcomponents C1r/C1s domain. Both the full length protein and C-terminal fragment can bind to the transforming growth factor beta type II receptor to promote the epithelial-mesenchymal transition and tumor angiogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

SCUBE3: Binds to TGFBR2 and activates TGFB signaling. In lung cancer cells, could serve as an endogenous autocrine and paracrine ligand of TGFBR2, which could regulate TGFBR2 signaling and hence modulate epithelial-mesenchymal transition and cancer progression. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; extracellular region

Molecular Function: protein binding; calcium ion binding

Biological Process: protein heterooligomerization; protein homooligomerization