Rabbit anti-Human, Mouse SCUBE3 Polyclonal Antibody | anti-SCUBE3 antibody

SCUBE3, ID (SCUBE3, CEGF3, Signal peptide, CUB and EGF-like domain-containing protein 3) (AP)

Cat. Num. AAA6345448

• Gene Names: SCUBE3; CEGF3
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SCUBE3; Polyclonal Antibody; ID (SCUBE3; CEGF3; Signal peptide; CUB and EGF-like domain-containing protein 3) (AP); CUB and EGF-like domain-containing protein 3; anti-SCUBE3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-SCUBE3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SCUBE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 480-509 amino acids from the Central region of human SCUBE3.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SCUBE3 Antibody (Center) western blot analysis in Uterus tissue lysates (35ug/lane).This demonstrates the SCUBE3 antibody detected the SCUBE3 protein (arrow).)

Western Blot (WB)

(SCUBE3 Antibody (Center) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the SCUBE3 antibody detected the SCUBE3 protein (arrow).)

Related Product Information for anti-SCUBE3 antibody

SCUBE3 contains one CUB domain and 9 EGF-like domains. It is highly expressed in primary osteoblasts and the long bones (humerus and femur) and expression is low or absent in non-bone tissues. It may play an important role in bone cell biology.

Product Categories/Family for anti-SCUBE3 antibody

Antibodies; Signal Transduction

References

Zhao, J., et al. BMC Med. Genet. 11, 96 (2010): Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008). Wu, B.T., et al. J. Biol. Chem. 279(36):37485-37490(2004). Mungall, A.J., et al. Nature 425(6960):805-811(2003). Yang, R.B., et al. J. Biol. Chem. 277(48):46364-46373(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 31377568
• NCBI GeneID: 222663
• NCBI Accession #: NP_689966.2
• NCBI GenBank Nucleotide #: NM_152753.3
• UniProt Accession #: Q8IX30; Q5CZB3; Q86UZ9; Q8NAU9; A8K5A3
• Molecular Weight: 111,099 Da
• NCBI Official Full Name: signal peptide, CUB and EGF-like domain-containing protein 3 isoform 1
• NCBI Official Synonym Full Names: signal peptide, CUB domain, EGF-like 3
• NCBI Official Symbol: SCUBE3
• NCBI Official Synonym Symbols: CEGF3
• NCBI Protein Information: signal peptide, CUB and EGF-like domain-containing protein 3; CUB domain and EGF-like repeat containing 3; signal peptide, CUB and EGF-like domain containing protein 3; signal peptide-, CUB domain-, and EGF-like domains-containing protein 3
• UniProt Protein Name: Signal peptide, CUB and EGF-like domain-containing protein 3
• Protein Family: Signal peptide, CUB and EGF-like domain-containing protein
• UniProt Gene Name: SCUBE3
• UniProt Entry Name: SCUB3_HUMAN

NCBI Description

This gene encodes a member of the signal peptide, complement subcomponents C1r/C1s, Uegf, bone morphogenetic protein-1 and epidermal growth factor-like domain containing protein family. Overexpression of this gene in human embryonic kidney cells results in secretion of a glycosylated form of the protein that forms oligomers and tethers to the cell surface. This gene is upregulated in lung cancer tumor tissue compared to healthy tissue and is associated with loss of the epithelial marker E-cadherin and with increased expression of vimentin, a mesenchymal marker. In addition, the protein encoded by this gene is a transforming growth factor beta receptor ligand, and when secreted by cancer cells, it can be cleaved in vitro to release the N-terminal epidermal growth factor-like repeat domain and the C-terminal complement subcomponents C1r/C1s domain. Both the full length protein and C-terminal fragment can bind to the transforming growth factor beta type II receptor to promote the epithelial-mesenchymal transition and tumor angiogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

SCUBE3: Binds to TGFBR2 and activates TGFB signaling. In lung cancer cells, could serve as an endogenous autocrine and paracrine ligand of TGFBR2, which could regulate TGFBR2 signaling and hence modulate epithelial-mesenchymal transition and cancer progression. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; extracellular region

Molecular Function: protein binding; calcium ion binding

Biological Process: protein heterooligomerization; protein homooligomerization