Goat SLC47A2 Polyclonal Antibody | anti-SLC47A2 antibody

Goat Polyclonal to Human SLC47A2

Cat. Num. AAA248219

• Gene Names: SLC47A2; MATE2; MATE2K; MATE2-B; MATE2-K
• Reactivity: Gorilla, Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: SLC47A2; Polyclonal Antibody; Goat Polyclonal to Human SLC47A2; Anti-SLC47A2 Antibody (C-Terminus) IHC-plus; MATE2; MATE2-K; H+/organic cation antiporter; HMATE-2; MATE-2; MATE2-B; MATE2K; Human SLC47A2; anti-SLC47A2 antibody

• Host: Goat
• Reactivity: Gorilla, Human
• Clonality: Polyclonal
• Specificity: Human SLC47A2. This antibody is expected to recognize both reported isoforms (NP_690872.2; NP_001093116.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 566

• Applicable Applications for anti-SLC47A2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (5 ug/ml), WB (0.1 - 1 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 60kD band observed in Human Testis lysates (calculated MW of 61.0kD according to NP_001093116.1). Recommended concentration: 0.1-1 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide SECHVDFFRTPEEAH from an internal region (near C-terminus) of human SLC47A2 (NP_690872.2; NP_001093116.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Chimpanzee, Orangutan (93%); Gibbon, Monkey, Marmoset (87%).
• Immunogen Type: Synthetic peptide
• Immunogen: SLC47A2 antibody was raised against synthetic peptide SECHVDFFRTPEEAH from an internal region (near C-terminus) of human SLC47A2 (NP_690872.2; NP_001093116.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Chimpanzee, Orangutan (93%); Gibbon, Monkey, Marmoset (87%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Human, Kidney: Formalin-Fixed Paraffin-Embedded (FFPE))

Western Blot (WB)

(SLC47A2 antibody (0.3 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 153792564
• NCBI GeneID: 146802
• NCBI Accession #: NP_001093116.1
• NCBI GenBank Nucleotide #: NM_001099646.1
• UniProt Accession #: Q86VL8; Q63HJ9; Q8IV44; Q96NA1; A0JBX9; A0P8Z7
• Molecular Weight: 23,344 Da
• NCBI Official Full Name: multidrug and toxin extrusion protein 2 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 47 (multidrug and toxin extrusion), member 2
• NCBI Official Symbol: SLC47A2
• NCBI Official Synonym Symbols: MATE2; MATE2K; MATE2-B; MATE2-K
• NCBI Protein Information: multidrug and toxin extrusion protein 2; MATE-2; hMATE-2; H+/organic cation antiporter; multidrug and toxin extrusion 2; solute carrier family 47, member 2; kidney-specific H(+)/organic cation antiporter
• UniProt Protein Name: Multidrug and toxin extrusion protein 2
• Protein Family: Multidrug and toxin extrusion protein
• UniProt Gene Name: SLC47A2
• UniProt Synonym Gene Names: MATE2; MATE-2; hMATE-2
• UniProt Entry Name: S47A2_HUMAN

NCBI Description

This gene encodes a protein belonging to a family of transporters involved in excretion of toxic electrolytes, both endogenous and exogenous, through urine and bile. This transporter family shares homology with the bacterial MATE (multidrug and toxin extrusion) protein family responsible for drug resistance. This gene is one of two members of the MATE transporter family located near each other on chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC47A2: Solute transporter for tetraethylammonium (TEA), 1- methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide, metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, and ganciclovir. Responsible for the secretion of cationic drugs across the brush border membranes. Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: plasma membrane; integral to membrane

Molecular Function: drug transporter activity; antiporter activity

Biological Process: multidrug transport; transmembrane transport