Goat HnRNP-E1 / PCBP1 Polyclonal Antibody | anti-HnRNP-E1 antibody

Goat Polyclonal to Human HnRNP-E1 / PCBP1

Cat. Num. AAA246993

• Gene Names: PCBP1; HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1
• Reactivity: Human; Predicted Reactivity: Gorilla, Gibbon, Mouse, Bat, Bovine, Dog, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: HnRNP-E1 / PCBP1; Polyclonal Antibody; Goat Polyclonal to Human HnRNP-E1 / PCBP1; Anti-HnRNP-E1 / PCBP1 Antibody (Internal) IHC-plus; PCBP1; Alpha-CP1; HnRNP E1; HnRNP-E1; HnRNP-X; HNRPE1; Poly(rC) binding protein 1; HNRPX; Poly(rC)-binding protein 1; Human HnRNP-E1; anti-HnRNP-E1 antibody

• Host: Goat
• Reactivity: Human; Predicted Reactivity: Gorilla, Gibbon, Mouse, Bat, Bovine, Dog, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human PCBP1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 356

• Applicable Applications for anti-HnRNP-E1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (5 ug/ml), WB (0.01 - 0.03 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 38kD band observed in lysates of cell line HeLa and in Human Placenta lysates (calculated MW of 37.5kD according to NP_006187.2). Recommended concentration: 0.01-0.03 ug/m...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-ADLAKLNQVARQQSH from an internal region of human PCBP1 (NP_006187.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit (93%); Monkey, Marmoset (87%).
• Immunogen Type: Synthetic peptide
• Immunogen: HnRNP-E1 / PCBP1 antibody was raised against synthetic peptide C-ADLAKLNQVARQQSH from an internal region of human PCBP1 (NP_006187.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit (93%); Monkey, Marmoset (87%).
• Antigen Modification: Internal
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-PCBP1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(PCBP1 antibody (0.01 ug/ml) staining of HeLa lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 222352151
• NCBI GeneID: 5093
• NCBI Accession #: NP_006187.2
• NCBI GenBank Nucleotide #: NM_006196.3
• UniProt Accession #: Q15365; Q13157; Q14975
• Molecular Weight: 37,498 Da
• NCBI Official Full Name: poly(rC)-binding protein 1
• NCBI Official Synonym Full Names: poly(rC) binding protein 1
• NCBI Official Symbol: PCBP1
• NCBI Official Synonym Symbols: HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1
• NCBI Protein Information: poly(rC)-binding protein 1; poly(rC)-binding protein 1; alpha-CP1; epididymis secretory protein Li 85; nucleic acid-binding protein SUB2.3; heterogenous nuclear ribonucleoprotein X; heterogenous nuclear ribonucleoprotein E1; heterogeneous nuclear ribonucleoprotein E1
• UniProt Protein Name: Poly(rC)-binding protein 1
• UniProt Gene Name: PCBP1
• UniProt Synonym Gene Names: hnRNP E1
• UniProt Entry Name: PCBP1_HUMAN

NCBI Description

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP E1: Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.

Protein type: Translation; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 2p13-p12

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; membrane; cytoplasm; ribonucleoprotein complex

Molecular Function: protein binding; RNA binding; single-stranded DNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression