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Western Blot (WB) (RDHE2 rabbit polyclonal antibody. Western Blot analysis of RDHE2 expression in mouse liver.)

Rabbit anti-Human, Mouse SDR16C5 Polyclonal Antibody | anti-SDR16C5 antibody

SDR16C5 (Short-chain Dehydrogenase/reductase Family 16C Member 5, Epidermal Retinol Dehydrogenase 2, EPHD-2, RDHE2, RDH-E2, RDH#2, Retinal Short-chain Dehydrogenase Reductase 2, RetSDR2) (HRP)

Gene Names
SDR16C5; RDH#2; RDHE2; RDH-E2
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SDR16C5; Polyclonal Antibody; SDR16C5 (Short-chain Dehydrogenase/reductase Family 16C Member 5; Epidermal Retinol Dehydrogenase 2; EPHD-2; RDHE2; RDH-E2; RDH#2; Retinal Short-chain Dehydrogenase Reductase 2; RetSDR2) (HRP); EC=1.1.1.105; anti-SDR16C5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human RDHE2. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-SDR16C5 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human RDHE2, aa1-309 (NP_620419.2).
Immunogen Sequence
MSFNLQSSKKLFIFLGKSLFSLLEAMIFALLPKPRKNVAGEIVLITGAGSGLGRLLALQFARLGSVLVLWDINKEGNEETCKMAREAGATRVHAYTCDCSQKEGVYRVADQVKKEVGDVSILINNAGIVTGKKFLDCPDELMEKSFDVNFKAHLWTYKAFLPAMIANDHGHLVCISSSAGLSGVNGLADYCASKFAAFGFAESVFVETFVQKQKGIKTTIVCPFFIKTGMFEGCTTGCPSLLPILEPKYAVEKIVEAILQEKMYLYMPKLLYFMMFLKSFLPLKTGLLIADYLGILHAMDGFVDQKKKL
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RDHE2 rabbit polyclonal antibody. Western Blot analysis of RDHE2 expression in mouse liver.)

Western Blot (WB) (RDHE2 rabbit polyclonal antibody. Western Blot analysis of RDHE2 expression in mouse liver.)

Western Blot (WB)

(Western Blot analysis of SDR16C5 expression in transfected 293T cell line by SDR16C5 polyclonal antibody. Lane 1: RDHE2 transfected lysate (34.1kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of SDR16C5 expression in transfected 293T cell line by SDR16C5 polyclonal antibody. Lane 1: RDHE2 transfected lysate (34.1kD). Lane 2: Non-transfected lysate.)
Product Categories/Family for anti-SDR16C5 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,164 Da
NCBI Official Full Name
epidermal retinol dehydrogenase 2
NCBI Official Synonym Full Names
short chain dehydrogenase/reductase family 16C, member 5
NCBI Official Symbol
SDR16C5
NCBI Official Synonym Symbols
RDH#2; RDHE2; RDH-E2
NCBI Protein Information
epidermal retinol dehydrogenase 2; EPHD-2; epidermal retinal dehydrogenase 2; retSDR2; retinal short chain dehydrogenase reductase; retinal short-chain dehydrogenase reductase 2; short-chain dehydrogenase/reductase family 16C member 5
UniProt Protein Name
Epidermal retinol dehydrogenase 2
UniProt Gene Name
SDR16C5
UniProt Synonym Gene Names
RDHE2; EPHD-2; RDH-E2; retSDR2
UniProt Entry Name
RDHE2_HUMAN

NCBI Description

RDHE2 belongs to a family of short-chain alcohol dehydrogenases/reductases that catalyze the first and rate-limiting step that generates retinaldehyde from retinol (Matsuzaka et al., 2002 [PubMed 12372410]).[supplied by OMIM, Mar 2008]

Uniprot Description

RDHE2: Oxidoreductase with strong preference for NAD. Active in both the oxidative and reductive directions. Oxidizes all-trans- retinol in all-trans-retinaldehyde. No activity was detected with 11-cis-retinol or 11-cis-retinaldehyde as substrates with either NAD(+)/NADH or NADP(+)/NADPH. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Oxidoreductase; Membrane protein, integral; EC 1.1.1.105

Chromosomal Location of Human Ortholog: 8q12.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: retinol dehydrogenase activity

Biological Process: retinal metabolic process; retinol metabolic process; detection of light stimulus involved in visual perception; keratinocyte proliferation

Research Articles on SDR16C5

Similar Products

Product Notes

The SDR16C5 sdr16c5 (Catalog #AAA6392269) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SDR16C5 (Short-chain Dehydrogenase/reductase Family 16C Member 5, Epidermal Retinol Dehydrogenase 2, EPHD-2, RDHE2, RDH-E2, RDH#2, Retinal Short-chain Dehydrogenase Reductase 2, RetSDR2) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SDR16C5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SDR16C5 sdr16c5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SDR16C5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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