Goat NOX2 / gp91phox Polyclonal Antibody | anti-NOX2 antibody

Goat Polyclonal to Human NOX2 / gp91phox

Cat. Num. AAA246990

• Gene Names: CYBB; CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
• Reactivity: Gorilla, Human; Predicted Reactivity: Gibbon, Monkey, Dog (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: NOX2 / gp91phox; Polyclonal Antibody; Goat Polyclonal to Human NOX2 / gp91phox; Anti-NOX2 / gp91phox Antibody (Internal) IHC-plus; CYBB; Chronic granulomatous disease; CGD91-phox; Cytochrome b558 subunit beta; Cytochrome b(558) subunit beta; gp91-1; gp91-PHOX; p22 phagocyte B-cytochrome; AMCBX2; CGD; Cytochrome b-245 heavy chain; gp91PHOX; NADPH oxidase 2; NOX2; p91-PHOX; Human NOX2; gp91phox; anti-NOX2 antibody

• Host: Goat
• Reactivity: Gorilla, Human; Predicted Reactivity: Gibbon, Monkey, Dog (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human CYBB / gp91phox.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 570

• Applicable Applications for anti-NOX2 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (5 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-SYLNFARKRIKNP from an internal region of human CYBB / gp91phox (NP_000388.2). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey, Marmoset, Elephant, Dog (92%); Dolphin, Bat (85%).
• Immunogen Type: Synthetic peptide
• Immunogen: NOX2 / gp91phox antibody was raised against synthetic peptide C-SYLNFARKRIKNP from an internal region of human CYBB / gp91phox (NP_000388.2). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey, Marmoset, Elephant, Dog (92%); Dolphin, Bat (85%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-NOX2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-NOX2 antibody

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles.

NCBI and Uniprot Product Information

• NCBI GI #: 6996021
• NCBI GeneID: 1536
• NCBI Accession #: NP_000388.2
• NCBI GenBank Nucleotide #: NM_000397.3
• UniProt Accession #: P04839; Q2PP16; A8K138
• Molecular Weight: 65,336 Da
• NCBI Official Full Name: cytochrome b-245 heavy chain
• NCBI Official Synonym Full Names: cytochrome b-245, beta polypeptide
• NCBI Official Symbol: CYBB
• NCBI Official Synonym Symbols: CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
• NCBI Protein Information: cytochrome b-245 heavy chain; CGD91-phox; NADPH oxidase 2; p22 phagocyte B-cytochrome; cytochrome b558 subunit beta; neutrophil cytochrome b 91 kDa polypeptide; heme-binding membrane glycoprotein gp91phox; superoxide-generating NADPH oxidase heavy chain subunit
• UniProt Protein Name: Cytochrome b-245 heavy chain
• UniProt Gene Name: CYBB
• UniProt Synonym Gene Names: NOX2; Cytochrome b558 subunit beta
• UniProt Entry Name: CY24B_HUMAN

NCBI Description

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]

Uniprot Description

CYBB: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of granulomatous disease,chronic, X-linked (CGD). A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life- threatening bacterial/fungal infections. Defects in CYBB are a cause of mycobacteriosis atypical X-linked type 2 (AMCBX2). A rare condition characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections.

Protein type: Membrane protein, multi-pass; Oxidoreductase; Mitochondrial; EC 1.-.-.-; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp21.1

Cellular Component: Golgi apparatus; phagocytic vesicle membrane; cell soma; mitochondrion; rough endoplasmic reticulum; integral to plasma membrane; dendrite; plasma membrane; NADPH oxidase complex

Molecular Function: protein binding; electron carrier activity; FAD binding; protein heterodimerization activity; superoxide-generating NADPH oxidase activity; metal ion binding; heme binding; voltage-gated ion channel activity

Biological Process: respiratory burst; response to drug; interaction with host; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; ion transport; vascular endothelial growth factor receptor signaling pathway; inflammatory response; superoxide release; response to nutrient; hydrogen peroxide biosynthetic process

Disease: Immunodeficiency 34; Granulomatous Disease, Chronic, X-linked