Goat NCOA3 / SRC-3 / AIB1 Polyclonal Antibody | anti-NCOA3 antibody

Goat Polyclonal to Human NCOA3 / SRC-3 / AIB1

Cat. Num. AAA246720

• Gene Names: NCOA3; ACTR; AIB1; RAC3; SRC3; pCIP; AIB-1; CTG26; SRC-3; CAGH16; KAT13B; TNRC14; TNRC16; TRAM-1; bHLHe42
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Human, Monkey, Pig; Predicted Reactivity: Mouse, Rat, Horse, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: NCOA3 / SRC-3 / AIB1; Polyclonal Antibody; Goat Polyclonal to Human NCOA3 / SRC-3 / AIB1; Anti-NCOA3 / SRC-3 / AIB1 Antibody IHC-plus; NCOA3; ACTR; AIB1; BHLHe42; CAGH16; CBP-interacting protein; CTG26; AIB-1; P/CIP; TNRC14; TRAM-1; Nuclear receptor coactivator 3; PCIP; RAC-3; SRC3; TNRC16; KAT13B; NCoA-3; SRC-3; Human NCOA3; anti-NCOA3 antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Human, Monkey, Pig; Predicted Reactivity: Mouse, Rat, Horse, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human NCOA3. This antibody is expected to recognise both reported isoforms (NP_006525.2; NP_858045.1)
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1420

• Applicable Applications for anti-NCOA3 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (3.75 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-TNETQRQKSHT from an internal region of human NCOA3 (NP_006525.2; NP_858045.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Elephant, Panda, Dog, Bovine, Bat, Pig (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: NCOA3 / SRC-3 / AIB1 antibody was raised against synthetic peptide C-TNETQRQKSHT from an internal region of human NCOA3 (NP_006525.2; NP_858045.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Elephant, Panda, Dog, Bovine, Bat, Pig (100%); Mouse, Rat, Horse, Rabbit (91%); Opossum, Turkey, Platypus, Lizard (82%).
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-NCOA3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-NCOA3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Related Product Information for anti-NCOA3 antibody

The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation.

NCBI and Uniprot Product Information

• NCBI GI #: 32307124
• NCBI GeneID: 8202
• NCBI Accession #: NP_006525.2
• NCBI GenBank Nucleotide #: NM_006534.3
• UniProt Accession #: Q9Y6Q9; Q0VF45; Q5JYD9; Q5JYE0; Q9BR49; Q9UPC9; Q9UPG4; Q9UPG7; A4LAZ5
• Molecular Weight: 154,892 Da
• NCBI Official Full Name: nuclear receptor coactivator 3 isoform b
• NCBI Official Synonym Full Names: nuclear receptor coactivator 3
• NCBI Official Symbol: NCOA3
• NCBI Official Synonym Symbols: ACTR; AIB1; RAC3; SRC3; pCIP; AIB-1; CTG26; SRC-3; CAGH16; KAT13B; TNRC14; TNRC16; TRAM-1; bHLHe42
• NCBI Protein Information: nuclear receptor coactivator 3; CBP-interacting protein; receptor-associated coactivator 3; amplified in breast cancer 1 protein; steroid receptor coactivator protein 3; class E basic helix-loop-helix protein 42; thyroid hormone receptor activator molecule 1
• UniProt Protein Name: Nuclear receptor coactivator 3
• Protein Family: Nuclear receptor coactivator
• UniProt Gene Name: NCOA3
• UniProt Synonym Gene Names: AIB1; BHLHE42; RAC3; TRAM1; NCoA-3; AIB-1; pCIP; bHLHe42; RAC-3; SRC-3; TRAM-1
• UniProt Entry Name: NCOA3_HUMAN

NCBI Description

The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq, Mar 2010]

Uniprot Description

SRC-3: a histone acetyltransferase and nuclear receptor coactivator. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Activity is enhanced by various hormones and TNF. Acetylation by CREBBP may suppress coactivation of target genes by disrupting its association with nuclear receptors. Frequently amplified or overexpressed in breast and ovarian cancers. Five alternatively spliced isoforms have been described.

Protein type: EC 2.3.1.48; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis; Acetyltransferase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; protein dimerization activity; signal transducer activity; protein binding; histone acetyltransferase activity; androgen receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; transcription coactivator activity; protein N-terminus binding; chromatin binding; thyroid hormone receptor binding; nuclear hormone receptor binding

Biological Process: developmental growth; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; multicellular organism growth; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; vagina development; positive regulation of transcription from RNA polymerase II promoter; histone acetylation; cellular response to hormone stimulus; positive regulation of keratinocyte differentiation