Mouse anti-Human CA1 / Carbonic Anhydrase I Monoclonal Antibody | anti-CA1 antibody

Mouse Monoclonal [clone 9D6D7] (IgG1) to Human CA1 / Carbonic Anhydrase I

Cat. Num. AAA246384

• Gene Names: CA1; CAB; CA-I; Car1; HEL-S-11
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Ascites
• Synonyms: CA1 / Carbonic Anhydrase I; Monoclonal Antibody; Mouse Monoclonal [clone 9D6D7] (IgG1) to Human CA1 / Carbonic Anhydrase I; Anti-CA1 / Carbonic Anhydrase I Antibody (clone 9D6D7) IHC-plus; CA1; CAB; Carbonic anhydrase 1; CAR1; Carbonate dehydratase I; Carbonic dehydratase; CA-I; Carbonic anhydrase B; Carbonic anhydrase I; Human CA1; Carbonic Anhydrase I; anti-CA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9D6D7
• Specificity: Human CA1
• Purity/Purification: Ascites
• Form/Format: Ascites fluid, 0.03% sodium azide
• Sequence Length: 261

• Applicable Applications for anti-CA1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IHC-P (1:200 - 1:300), WB (1:500 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Purified recombinant fragment of CA1(aa25-90) expressed in E Coli.
• Immunogen: CA1 / Carbonic Anhydrase I antibody was raised against purified recombinant fragment of CA1(aa25-90) expressed in E Coli.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-CA1 / Carbonic Anhydrase I antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

Immunohistochemistry (IHC)

(Anti-CA1 / Carbonic Anhydrase I antibody IHC of human vessel, red blood cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

Immunohistochemistry (IHC)

(Anti-CA1 / Carbonic Anhydrase I antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

Western Blot (WB)

(Western blot of anti-CA1 monoclonal antibody against truncated Trx-CA1 recombinant protein, MBP-CA1(aa25-90) recombinant protein and full length CA1(aa1-261)-hIgGFc transfected CHO-K1 cell lysate(3).)

NCBI and Uniprot Product Information

• NCBI GI #: 4502517
• NCBI GeneID: 759
• NCBI Accession #: NP_001729.1
• NCBI GenBank Nucleotide #: NM_001738.4
• UniProt Accession #: P00915
• Molecular Weight: 28,870 Da
• NCBI Official Full Name: carbonic anhydrase 1 isoform a
• NCBI Official Synonym Full Names: carbonic anhydrase I
• NCBI Official Symbol: CA1
• NCBI Official Synonym Symbols: CAB; CA-I; Car1; HEL-S-11
• NCBI Protein Information: carbonic anhydrase 1; carbonic anhydrase B; carbonate dehydratase I; epididymis secretory protein Li 11
• UniProt Protein Name: Carbonic anhydrase 1
• Protein Family: Cation-transporting ATPase
• UniProt Gene Name: CA1
• UniProt Synonym Gene Names: CAB; CA-I
• UniProt Entry Name: CAH1_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; EC 4.2.1.1; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process