Mouse anti-Human, Mouse STAG2 Monoclonal Antibody | anti-STAG2 antibody

Mouse Monoclonal [clone 3C6] (IgG1,k) to Human STAG2

Cat. Num. AAA246117

• Gene Names: STAG2; SA2; SA-2; SCC3B; bA517O1.1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunofluorescence
• Purity: Purified from ascites by Protein A
• Synonyms: STAG2; Monoclonal Antibody; Mouse Monoclonal [clone 3C6] (IgG1; k) to Human STAG2; Anti-STAG2 Antibody (clone 3C6) IHC-plus; Cohesin subunit SA-2; SCC3B; SCC3 homolog 2; Stromal antigen 2; SA2; BA517O1.1; SA-2; Human STAG2; anti-STAG2 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3C6
• Specificity: Human Nuclear Protein SA-2
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1231

• Applicable Applications for anti-STAG2 antibody: Western Blot (WB), ELISA (EIA),Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF)
• Application Notes: Western Blot (WB); ELISA (optimal dilution to be determined by the researcher); IHC-P (5 ug/ml); Immunofluorescence (10ug/ml)
• Target Species: Human
• Antigen type: Recombinant Protein
• Immunogen: STAG2 antibody was raised against sTAG2 (NP_006594, 1130 a.a. ~ 1232 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only.; ; Tissue Preperation:; Formalin fixation and embedding in paraffin wax.; ; Tissue Sectioning; Make 4-um sections and place pre-cleaned and charged microscope slides. Heat in a tissue-drying oven for 45 minutes at 60°C.; ; Deparaffinization:; Wash dry slides in 3 changes of xylene - 5 minutes each at RT.; ; Rehydration:; Wash slides in 3 changes of 100% alcohol - 3 minutes each at RT.; Wash slides in 2 changes of 95% alcohol - 3 minutes each at RT.; Wash slides in 1 change of 80% alcohol - 3 minutes each at RT.; Rinse slides in gentle running distilled water - 5 minutes at RT; ; Antigen Retrieval; Steam slides in 0.01 M sodium cirate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at RT in buffer - 20 minutes; Rinse slides in 1X TBS with tween (TBST) - 1 minute at RT.; ; Immunostaining:; (Do not allow tissues to dry at any time during the staining procedure.); Apply a universal protein block - 20 minutes at RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes at RT; Rinse slides in 1X TBST - 1 minutes at RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes at RT; Rinse slides in 1X TBST - 1 minutes at RT; Appply alkaline phosphatase streptavidin - 30 minutes at RT; Rinse slides in 1X TBST - 1 minutes at RT; Apply alkaline phosphatase chromogen substrate - 30 minutes at RT; Wash slides in distilled water - 1 minute at RT; ; Dehydrate:; (This method should only be used if the chromogen substrate is alcohol insoulable (e.g Vector Red, DAB); Wash slides in 2 changes of 80% alcohol - 1 minute each at RT; Wash slides in 2 changes of 95% alcohol - 1 minute each at RT; Wash slides in 2 changes of 80% alcohol - 1 minute each at RT; Wash slides in 3 changes of 100% alcohol - 1 minute each at RT; Wash slides in 3 changes of xylene - 1 minute each at RT; Appply Covership
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-STAG2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to STAG2 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(Western blot of STAG2 expression in NIH/3T3 cells with STAG2 monoclonal antibody, clone 3C6.)

Western Blot (WB)

(Western blot of STAG2 expression in HeLa nuclear extract with STAG2 monoclonal antibody, clone 3C6.)

NCBI and Uniprot Product Information

• NCBI GI #: 31563531
• NCBI GeneID: 10735
• NCBI Accession #: NP_006594.3
• NCBI GenBank Nucleotide #: NM_006603.4
• UniProt Accession #: Q8N3U4; O00540; Q5JTI6; Q68DE9; Q9H1N8; B1AMT5; D3DTF5
• Molecular Weight: 145,751 Da
• NCBI Official Full Name: cohesin subunit SA-2 isoform b
• NCBI Official Synonym Full Names: stromal antigen 2
• NCBI Official Symbol: STAG2
• NCBI Official Synonym Symbols: SA2; SA-2; SCC3B; bA517O1.1
• NCBI Protein Information: cohesin subunit SA-2; SCC3 homolog 2
• UniProt Protein Name: Cohesin subunit SA-2
• Protein Family: Cofilin
• UniProt Gene Name: STAG2
• UniProt Synonym Gene Names: SA2
• UniProt Entry Name: STAG2_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

Uniprot Description

STAG2: Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1A or SMC1B) and SMC3, which are attached via their hinge domain, and RAD21 which link them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3. Belongs to the SCC3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; membrane; plasma membrane; nucleolus; chromosome; chromatin; nucleus; cytosol; chromosome, pericentric region; actin cytoskeleton

Molecular Function: protein binding; chromatin binding

Biological Process: mitosis; meiotic cell cycle; cell division; stem cell maintenance; sister chromatid cohesion; negative regulation of DNA endoreduplication; mitotic cell cycle