Mouse anti-Human HK1 / Hexokinase 1 Monoclonal Antibody | anti-HK1 antibody

Mouse Monoclonal [clone 4D7] (Ig2a,k) to Human HK1 / Hexokinase 1

Cat. Num. AAA245499

• Gene Names: HK1; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein G Purified
• Synonyms: HK1 / Hexokinase 1; Monoclonal Antibody; Mouse Monoclonal [clone 4D7] (Ig2a; k) to Human HK1 / Hexokinase 1; Anti-HK1 / Hexokinase 1 Antibody (aa1-917; clone 4D7) IHC-plus; HK1; Brain form hexokinase; Hexokinase 1; Hexokinase type I; HK I; HK1-tb; HK1-tc; HKI; Hexokinase type 1; HK1-ta; HXK1; Glycolytic enzyme; Hexokinase-1; Type 1 hexokinase; Type i hexokinase; Human HK1; anti-HK1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Ig2a,k
• Clone Number: 4D7
• Purity/Purification: Protein G Purified
• Form/Format: PBS, pH 7.4, 0.1% sodium azide. Sourced in Ascites.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 917

• Applicable Applications for anti-HK1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1:1000 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Recombinant human Hexokinase1 (1-917aa) purified from E Coli.
• Immunogen Type: Recombinant protein
• Immunogen: HK1 / Hexokinase 1 antibody was raised against recombinant human Hexokinase1 (1-917aa) purified from E Coli.
• Antigen Modification: aa1-917
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-HK1 / Hexokinase 1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Cell lysates of HepG2 (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection.)

Product Categories/Family for anti-HK1 antibody

Family: Non-Protein Kinase; Subfamily: Hexokinase

NCBI and Uniprot Product Information

• NCBI GI #: 188497754
• NCBI GeneID: 3098
• NCBI Accession #: NP_000179.2
• NCBI GenBank Nucleotide #: NM_000188.2
• UniProt Accession #: P19367; O43443; O43444; O75574; Q5VTC3; Q96HC8; Q9NNZ4; Q9NNZ5; E9PCK0
• Molecular Weight: 101,085 Da
• NCBI Official Full Name: hexokinase-1 isoform HKI
• NCBI Official Synonym Full Names: hexokinase 1
• NCBI Official Symbol: HK1
• NCBI Official Synonym Symbols: HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
• NCBI Protein Information: hexokinase-1; HK I; glycolytic enzyme; hexokinase type I; brain form hexokinase
• UniProt Protein Name: Hexokinase-1
• Protein Family: Probable histidine kinase
• UniProt Gene Name: HK1
• UniProt Synonym Gene Names: HK I
• UniProt Entry Name: HXK1_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]

Uniprot Description

HK1: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). HK-2 and its mitochondrial receptor (VDAC) play the most pivotal and direct roles in the ""Warburg effect"". Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Four human isoforms are produced by alternative splicing and alternative initiation. Isoform HK1 is markedly elevated in rapidly growing tumor cells exhibiting high glucose catabolic rates. HK1 is present in most tissues but is especially prominent in brain and kidney. Isoform HK1-SC is is an integral membrane protein detected in round spermatids, condensing spermatids and mature sperm where it is found in the head membranes, mitochondria of the midpiece and the fibrous sheath of the flagellum. Isoform HK1-SA is first expressed during meiosis and continues to be present in postmeiotic germ cells while isoform HK1-SB is present only in postmeiotic germ cells.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - starch and sucrose; EC 2.7.1.1; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - galactose; Mitochondrial; Kinase, other

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol; lipid raft

Molecular Function: hexokinase activity; protein binding; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding

Biological Process: hexose metabolic process; glycolysis; hexose transport; glucose 6-phosphate metabolic process; carbohydrate metabolic process; carbohydrate phosphorylation; cell glucose homeostasis; pathogenesis; glucose transport; transmembrane transport

Disease: Hemolytic Anemia, Nonspherocytic, Due To Hexokinase Deficiency; Neuropathy, Hereditary Motor And Sensory, Russe Type