Porcine Hexokinase 1 ELISA Kit | HK1 elisa kit

Porcine Hexokinase 1 ELISA Kit

Cat. Num. AAA737505

• Gene Names: HK1; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
• Reactivity: Porcine
• Synonyms: Hexokinase 1; Porcine Hexokinase 1 ELISA Kit; HK1 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 U/L.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HK1 elisa kit

Intended Uses: This HK1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine HK1. This ELISA kit for research use only!

Principle of the Assay: HK1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HK1 antibody and an HK1-HRP conjugate. The assay sample and buffer are incubated together with HK1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HK1 concentration since HK1 from samples and HK1-HRP conjugate compete for the anti-HK1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HK1 from the sample, fewer sites are left to bind HK1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HK1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HK1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 188497754
• NCBI GeneID: 3098
• NCBI Accession #: NP_000179.2
• NCBI GenBank Nucleotide #: NM_000188.2
• Molecular Weight: 101,085 Da
• NCBI Official Full Name: hexokinase-1 isoform HKI
• NCBI Official Synonym Full Names: hexokinase 1
• NCBI Official Symbol: HK1
• NCBI Official Synonym Symbols: HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
• NCBI Protein Information: hexokinase-1; HK I; glycolytic enzyme; hexokinase type I; brain form hexokinase
• UniProt Protein Name: Hexokinase-1
• Protein Family: Probable histidine kinase
• UniProt Gene Name: HK1
• UniProt Synonym Gene Names: HK I
• UniProt Entry Name: HXK1_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]

Uniprot Description

HK1: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). HK-2 and its mitochondrial receptor (VDAC) play the most pivotal and direct roles in the ""Warburg effect"". Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Four human isoforms are produced by alternative splicing and alternative initiation. Isoform HK1 is markedly elevated in rapidly growing tumor cells exhibiting high glucose catabolic rates. HK1 is present in most tissues but is especially prominent in brain and kidney. Isoform HK1-SC is is an integral membrane protein detected in round spermatids, condensing spermatids and mature sperm where it is found in the head membranes, mitochondria of the midpiece and the fibrous sheath of the flagellum. Isoform HK1-SA is first expressed during meiosis and continues to be present in postmeiotic germ cells while isoform HK1-SB is present only in postmeiotic germ cells.

Protein type: EC 2.7.1.1; Mitochondrial; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol; lipid raft

Molecular Function: protein binding; hexokinase activity; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding

Biological Process: hexose metabolic process; glycolysis; hexose transport; glucose 6-phosphate metabolic process; carbohydrate phosphorylation; carbohydrate metabolic process; cell glucose homeostasis; pathogenesis; glucose transport; transmembrane transport

Disease: Hemolytic Anemia, Nonspherocytic, Due To Hexokinase Deficiency; Neuropathy, Hereditary Motor And Sensory, Russe Type