Mouse TLR9 Monoclonal Antibody | anti-TLR9 antibody

Mouse Monoclonal [clone 26C593.2] (IgG1,k) to Human TLR9

Cat. Num. AAA245240

• Gene Names: TLR9; CD289
• Reactivity: Gibbon, Gorilla, Human, Mouse, Orangutan, Rat; Predicted Reactivity: Chimpanzee, Monkey (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein G Purified
• Synonyms: TLR9; Monoclonal Antibody; Mouse Monoclonal [clone 26C593.2] (IgG1; k) to Human TLR9; Anti-TLR9 Antibody (aa268-284; clone 26C593.2) IHC-plus; CD289; CD289 antigen; Scri2a; Toll-like receptor 9; Human TLR9; anti-TLR9 antibody

• Host: Mouse
• Reactivity: Gibbon, Gorilla, Human, Mouse, Orangutan, Rat; Predicted Reactivity: Chimpanzee, Monkey (at least 90% immunogen sequence identity)
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 26C593.2
• Specificity: KLH-conjugated synthetic peptide corresponding to amino acids 268-284 of human TLR9 isoform A (Genbank accession no. AAF78037).
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.05% BSA, 0.05% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1032

• Applicable Applications for anti-TLR9 antibody: Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo, IHC-Fr (10 - 20 ug/ml), IHC-P (5 ug/ml), WB (1 - 3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-284 (CPRHFPQLHPDTFSHLS) of human TLR9 isoform A (Genbank accession no. AAF78037).
• Immunogen Type: Synthetic peptide - KLH conjugated
• Immunogen: TLR9 antibody was raised against this antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-284 (CPRHFPQLHPDTFSHLS) of human TLR9 isoform A (Genbank accession no. AAF78037). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Chimpanzee, Marmoset (94%); Sheep, Goat, Zebu, Bovine, Horse (82%).
• Antigen Modification: aa268-284
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TLR9 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Flow Cytometry (FC/FACS)

(Intracellular flow analysis of TLR9 in Ramos cells using 0.1 ug of antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control; red represents anti-TLR9 antibody.)

Western Blot (WB)

(Western blot of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using antibody at a dilution of 3 ug/ml. Lane E shows antibody tested at 5 ug/ml in human MCF7 cell lysate.)

Product Categories/Family for anti-TLR9 antibody

Family: Toll-like Receptor

NCBI and Uniprot Product Information

• NCBI GI #: 8394456
• NCBI GeneID: 54106
• NCBI Accession #: NP_059138.1
• NCBI GenBank Nucleotide #: NM_017442.3
• UniProt Accession #: Q9NR96; Q6UVZ2; Q9HD68; Q9HD69; Q9HD70; Q9NYC2; Q9NYC3; B3Y661; D1CS56
• Molecular Weight: 114,237 Da
• NCBI Official Full Name: toll-like receptor 9
• NCBI Official Synonym Full Names: toll-like receptor 9
• NCBI Official Symbol: TLR9
• NCBI Official Synonym Symbols: CD289
• NCBI Protein Information: toll-like receptor 9
• UniProt Protein Name: Toll-like receptor 9
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR9
• UniProt Entry Name: TLR9_HUMAN