Mouse SYK Monoclonal Antibody | anti-SYK antibody

Mouse Monoclonal [clone SYK-01] (IgG1) to Human SYK

Cat. Num. AAA245128

• Gene Names: SYK; p72-Syk
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation
• Purity: Protein A Purified
• Synonyms: SYK; Monoclonal Antibody; Mouse Monoclonal [clone SYK-01] (IgG1) to Human SYK; Anti-SYK Antibody (aa5-360; clone SYK-01) IHC-plus; p72syk; Spleen tyrosine kinase; Syk protein-tyrosine kinase; Tyrosine-protein kinase SYK; p72-Syk; Human SYK; anti-SYK antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SYK-01
• Specificity: Recombinant fragment (aa 5-360) of human Syk
• Purity/Purification: Protein A Purified
• Form/Format: PBS, 15 mM sodium azide, approx., pH 7.4.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 635

• Applicable Applications for anti-SYK antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: ICC, IHC-P (5 ug/ml), IP, WB (1 - 2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Recombinant human SYK.
• Immunogen Type: Recombinant protein
• Immunogen: SYK antibody was raised against recombinant human SYK.
• Antigen Modification: aa5-360
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-SYK antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Product Categories/Family for anti-SYK antibody

Family: Protein Kinase; Subfamily: Syk/ZAP70

NCBI and Uniprot Product Information

• NCBI GI #: 21361553
• NCBI GeneID: 6850
• NCBI Accession #: NP_003168.2
• NCBI GenBank Nucleotide #: NM_003177.6
• UniProt Accession #: P43405
• Molecular Weight: 69,510 Da
• NCBI Official Full Name: tyrosine-protein kinase SYK isoform Syk(L)
• NCBI Official Synonym Full Names: spleen tyrosine kinase
• NCBI Official Symbol: SYK
• NCBI Official Synonym Symbols: p72-Syk
• NCBI Protein Information: tyrosine-protein kinase SYK
• UniProt Protein Name: Tyrosine-protein kinase SYK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: SYK
• UniProt Entry Name: KSYK_HUMAN

NCBI Description

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.

Protein type: EC 2.7.10.2; Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Syk family

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; protein complex; cytoplasm; plasma membrane; nucleus; cytosol; B cell receptor complex

Molecular Function: integrin binding; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: viral reproduction; transcription factor import into nucleus; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of interleukin-3 biosynthetic process; positive regulation of calcium-mediated signaling; regulation of phagocytosis; protein amino acid phosphorylation; activation of JNK activity; B cell receptor signaling pathway; leukocyte adhesion; regulation of transcription factor activity; positive regulation of bone resorption; beta selection; angiogenesis; positive regulation of gamma-delta T cell differentiation; inflammatory response; integrin-mediated signaling pathway; platelet activation; neutrophil chemotaxis; adaptive immune response; regulation of superoxide release; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; regulation of neutrophil degranulation; leukotriene biosynthetic process; cell proliferation; organ morphogenesis; positive regulation of peptidyl-tyrosine phosphorylation; neutrophil activation during immune response; leukocyte activation during immune response; serotonin secretion by platelet; defense response to bacterium; macrophage activation during immune response; blood vessel morphogenesis; positive regulation of B cell differentiation; innate immune response; positive regulation of alpha-beta T cell proliferation; blood coagulation; lymph vessel development; positive regulation of alpha-beta T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cytokine secretion