Goat P2RX7 / P2X7 Polyclonal Antibody | anti-P2RX7 antibody

Goat Polyclonal to Human P2RX7 / P2X7

Cat. Num. AAA244892

• Gene Names: P2RX7; P2X7
• Reactivity: Gibbon, Gorilla, Hamster, Human, Pig; Predicted Reactivity: Monkey, Mouse, Rat, Guinea pig (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: P2RX7 / P2X7; Polyclonal Antibody; Goat Polyclonal to Human P2RX7 / P2X7; Anti-P2RX7 / P2X7 Antibody (N-Terminus) IHC-plus; P2RX7; ATP receptor; p2X purinoceptor 7; p2X receptor 7; p2X7 purinoceptor; p2Z receptor; Purinergic receptor; p2RX7; p2X7; p2X7 receptor; Human P2RX7; P2X7; anti-P2RX7 antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Hamster, Human, Pig; Predicted Reactivity: Monkey, Mouse, Rat, Guinea pig (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human P2RX7 / P2X7.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 595

• Applicable Applications for anti-P2RX7 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (3.75 ug/ml), WB (1 - 3 ug/ml); Usage: Western Blot: Approx 37kD and 70kD band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 68.6kD according to NP_002553.2). Recommended concentration: 1-3 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide YETNKVTRIQSMNY-C from the N-terminus of human P2RX7 / P2X7 (NP_002553.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Hamster, Pig (100%); Monkey, Mouse, Rat, Guinea pig (93%).
• Immunogen Type: Synthetic peptide
• Immunogen: P2RX7 / P2X7 antibody was raised against synthetic peptide YETNKVTRIQSMNY-C from the N-terminus of human P2RX7 / P2X7 (NP_002553.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Hamster, Pig (100%); Monkey, Mouse, Rat, Guinea pig (93%); Elephant, Panda, Dog, Rabbit (86%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-P2RX7 / P2X7 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-P2RX7 / P2X7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(P2RX7 antibody staining (0.3 ug/ml) of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-P2RX7 antibody

P2X7 is a member of a family of P2X receptors that are fast, ligand-gated cation channels that differ in the length of their intracellular C-termini. The long C-terminus of P2X7 allows time-dependent dilation of the channel, in response to ATP, to form a cytolytic pore allowing entry of large molecules.

Product Categories/Family for anti-P2RX7 antibody

Family: Ion Channel; Subfamily: P2X receptor

NCBI and Uniprot Product Information

• NCBI GI #: 29294631
• NCBI GeneID: 5027
• NCBI Accession #: NP_002553.2
• NCBI GenBank Nucleotide #: NM_002562.4
• UniProt Accession #: Q99572; O14991; Q4VKH8; Q4VKH9; Q4VKI0; Q4VKI1; Q4VKI2; A8K2Z0; E7EMK6; F5H6P2; F5H7E8; F8W951
• Molecular Weight: 58,239 Da
• NCBI Official Full Name: P2X purinoceptor 7
• NCBI Official Synonym Full Names: purinergic receptor P2X, ligand-gated ion channel, 7
• NCBI Official Symbol: P2RX7
• NCBI Official Synonym Symbols: P2X7
• NCBI Protein Information: P2X purinoceptor 7; ATP receptor; P2Z receptor; P2X7 receptor; purinergic receptor P2X7 variant A
• UniProt Protein Name: P2X purinoceptor 7
• Protein Family: P2X purinoceptor
• UniProt Gene Name: P2RX7
• UniProt Synonym Gene Names: P2X7
• UniProt Entry Name: P2RX7_HUMAN

NCBI Description

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]

Uniprot Description

P2X7: a receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis by macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Alternatively spliced variants encoding different isoforms have been identified. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. Phosphorylation results in its inactivation.

Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: protein complex; membrane; cell soma; integral to plasma membrane; cytoplasm; plasma membrane; terminal button; integral to nuclear inner membrane; intercellular junction; neuromuscular junction; external side of plasma membrane; lipid raft

Molecular Function: protein binding; protein homodimerization activity; copper ion binding; zinc ion binding; lipopolysaccharide binding; magnesium ion binding; ATP binding; purinergic nucleotide receptor activity; receptor binding; ATP-gated cation channel activity

Biological Process: positive regulation of T cell mediated cytotoxicity; activation of MAPK activity; cytolysis; positive regulation of glutamate secretion; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; sensory perception of pain; protein amino acid phosphorylation; positive regulation of interleukin-1 alpha secretion; cell surface receptor linked signal transduction; multicellular organismal protein catabolic process; ceramide biosynthetic process; positive regulation of cytolysis; T cell homeostasis; response to electrical stimulus; positive regulation of gamma-aminobutyric acid secretion; response to drug; mitochondrion organization and biogenesis; collagen metabolic process; positive regulation of interleukin-6 production; positive regulation of cytoskeleton organization and biogenesis; regulation of sodium ion transport; protein oligomerization; phospholipid translocation; phagolysosome formation; membrane depolarization; defense response to Gram-positive bacterium; NAD transport; response to mechanical stimulus; response to zinc ion; response to calcium ion; cell volume homeostasis; phospholipid transfer to membrane; generation of action potential; cell morphogenesis; regulation of killing of cells of another organism; T cell proliferation; plasma membrane organization and biogenesis; positive regulation of mitochondrial depolarization; pore complex biogenesis; inflammatory response; negative regulation of bone resorption; negative regulation of MAPKKK cascade; membrane protein ectodomain proteolysis; bleb formation; positive regulation of bone mineralization; response to ATP; synaptic vesicle exocytosis; homeostasis of number of cells within a tissue; skeletal morphogenesis; release of sequestered calcium ion into cytosol; positive regulation of prostaglandin secretion; cellular response to extracellular stimulus; innate immune response; gene expression; protein processing; membrane budding

Disease: Leukemia, Chronic Lymphocytic