Mouse anti-Human FBL Monoclonal Antibody | anti-FBL antibody

Mouse monoclonal antibody Anti-Human FBL

Cat. Num. AAA120194

• Gene Names: FBL; FIB; FLRN; RNU3IP1
• Reactivity: Human
• Applications: Dot Blot
• Synonyms: FBL; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human FBL; Homo sapiens fibrillarin ; FIB; FLRN; RNU3IP1; anti-FBL antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2350C1
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 1171

• Applicable Applications for anti-FBL antibody: Dot Blot (DB)
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-FBL monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Related Product Information for anti-FBL antibody

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 215272338
• NCBI GeneID: 2091
• NCBI Accession #: NP_001427.2
• NCBI GenBank Nucleotide #: NM_001436
• Molecular Weight: 33,784 Da
• NCBI Official Full Name: Homo sapiens fibrillarin (FBL), mRNA
• NCBI Official Synonym Full Names: fibrillarin
• NCBI Official Symbol: FBL
• NCBI Official Synonym Symbols: FIB; FLRN; RNU3IP1
• NCBI Protein Information: rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; RNA, U3 small nucleolar interacting protein 1; histone-glutamine methyltransferase
• UniProt Protein Name: rRNA 2'-O-methyltransferase fibrillarin
• Protein Family: rRNA 2'-O-methyltransferase fibrillarin
• UniProt Gene Name: FBL
• UniProt Entry Name: FBRL_HUMAN

NCBI Description

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]

Uniprot Description

FBL: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Interacts with NOLC1. Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5. Belongs to the methyltransferase superfamily. Fibrillarin family.

Protein type: RNA processing; RNA-binding; Methyltransferase; Nucleolus; EC 2.1.1.-

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: Cajal body; granular component; membrane; nucleolus; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: osteoblast differentiation; tRNA processing; snoRNA metabolic process; rRNA processing