Rabbit STIM2 Polyclonal Antibody | anti-STIM2 antibody

Rabbit Polyclonal (IgG) to Human STIM2

Cat. Num. AAA244150

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: STIM2; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human STIM2; Anti-STIM2 Antibody (Internal) IHC-plus; KIAA1482; Stromal interaction molecule 2; Human STIM2; anti-STIM2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: a 17 amino acid peptide from near the center of human STIM2
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 191154276

• Applicable Applications for anti-STIM2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human STIM2.
• Immunogen Type: Synthetic peptide
• Immunogen: STIM2 antibody was raised against synthetic peptide from human STIM2.
• Antigen Modification: Internal
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-STIM2 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-STIM2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-STIM2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of STIM2 in Human Spleen cells with STIM2 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of STIM2 in A-20 cell lysate with at (A) 0.5, (B) 1 and (C) 2 ug/ml.)

Related Product Information for anti-STIM2 antibody

This gene is a member of the stromal interaction molecule (STIM) family and likely arose, with related family member STIM1, from a common ancestral gene. It encodes a type 1 transmembrane protein whose function is currently unknown, but is a likely adhesion molecule with a role in early hematopoiesis. Alternative translation initiation from an AUG and a non-AUG (UUG) start site results in the production of two different isoforms.

NCBI and Uniprot Product Information

• NCBI GI #: 224589816
• NCBI GeneID: 57620
• NCBI Accession #: NC_000004.11
• NCBI GenBank Nucleotide #: NC_000004.11
• Molecular Weight: 68,026 Da
• NCBI Official Full Name: Homo sapiens chromosome 4, GRCh37.p13 Primary Assembly
• NCBI Official Synonym Full Names: stromal interaction molecule 2
• NCBI Official Symbol: STIM2
• NCBI Protein Information: stromal interaction molecule 2
• UniProt Protein Name: Stromal interaction molecule 2
• Protein Family: Stromal interaction molecule
• UniProt Gene Name: STIM2
• UniProt Synonym Gene Names: KIAA1482
• UniProt Entry Name: STIM2_HUMAN

NCBI Description

This gene is a member of the stromal interaction molecule (STIM) family and likely arose, along with related family member STIM1, from a common ancestral gene. The encoded protein functions to regulate calcium concentrations in the cytosol and endoplasmic reticulum, and is involved in the activation of plasma membrane Orai Ca(2+) entry channels. This gene initiates translation from a non-AUG (UUG) start site. A signal peptide is cleaved from the resulting protein. Multiple transcript variants result from alternative splicing. [provided by RefSeq, Dec 2009]

Uniprot Description

STIM2: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx. Oligomer with STIM1. Interacts with ORAI1. Expressed in all tissues and tumor cell lines examined.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane; plasma membrane

Molecular Function: protein binding; store-operated calcium channel activity; calcium channel regulator activity; calcium ion binding

Biological Process: cellular calcium ion homeostasis; activation of store-operated calcium channel activity; positive regulation of calcium ion transport