Rabbit RAB3A Polyclonal Antibody | anti-RAB3A antibody

Rabbit Polyclonal to Human RAB3A

Cat. Num. AAA242364

• Reactivity: Gorilla, Human, Monkey
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: RAB3A; Polyclonal Antibody; Rabbit Polyclonal to Human RAB3A; Anti-RAB3A Antibody (aa202-217) IHC-plus; RAS-associated protein RAB3A; Ras-related protein Rab-3A; Human RAB3A; anti-RAB3A antibody

• Host: Rabbit
• Reactivity: Gorilla, Human, Monkey
• Clonality: Polyclonal
• Specificity: Detects an ~25 kD protein, corresponding to the apparent molecular mass of Rab3A on SDSPAGE immunoblots, in samples from mouse and rat origins. The antibody specificity is confirmed in peptide inhibition immunoblotting studies.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Borate buffered saline, pH 8.2 (25mM sodium borate, 100mM boric acid, 75mM NaCl, 5mM EDTA (disodium salt)
• Concentration: 0.85 mg/ml (varies by lot)
• Sequence Length: 220

• Applicable Applications for anti-RAB3A antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1 - 2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: A 16 residue synthetic peptide (C)KQGPQLSDQQVPPHQD based on the human Rab3A (residues 202-217) with the cysteine (C) residue added and the peptide coupled to KLH.
• Immunogen Type: Synthetic peptide - KLH conjugated
• Immunogen: RAB3A antibody was raised against a 16 residue synthetic peptide (C)KQGPQLSDQQVPPHQD based on the human Rab3A (residues 202-217) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%); Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus, Xenopus (88%).
• Antigen Modification: aa202-217
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-RAB3A antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-RAB3A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Product Categories/Family for anti-RAB3A antibody

Family: Ras GTPase superfamily IPR001806; Subfamily: RAS oncogene

NCBI and Uniprot Product Information

• NCBI GI #: 4506367
• NCBI GeneID: 5864
• NCBI Accession #: NP_002857.1
• NCBI GenBank Nucleotide #: NM_002866.4
• UniProt Accession #: P20336; Q9NYE1; A8K0J4
• Molecular Weight: 24,984 Da
• NCBI Official Full Name: ras-related protein Rab-3A
• NCBI Official Synonym Full Names: RAB3A, member RAS oncogene family
• NCBI Official Symbol: RAB3A
• NCBI Protein Information: ras-related protein Rab-3A; RAS-associated protein RAB3A
• UniProt Protein Name: Ras-related protein Rab-3A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB3A
• UniProt Entry Name: RAB3A_HUMAN

Uniprot Description

RAB3A: Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. Heterodimer with RIMS2. Part of a ternary complex involving PCLO and EPAC2. Interacts with RPH3A. Interacts with the exocyst complex through SEC15. Binds SYTL4, RIMS1 and RIMS2. Interacts with RAB3IP. Interacts with SGSM1 and SGSM3. Specifically expressed in brain. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein; G protein, monomeric, Rab; G protein, monomeric

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: synaptic vesicle; protein complex; axon; secretory granule membrane; acrosome; plasma membrane; terminal button; cytosol; endosome; vesicle

Molecular Function: GTPase activity; protein C-terminus binding; ATPase activator activity; GTPase binding; protein binding; GDP binding; GTP binding; myosin V binding; ATPase binding

Biological Process: mitochondrion organization and biogenesis; synaptic vesicle maturation; metabolic process; neurotransmitter secretion; regulation of synaptic vesicle fusion to presynaptic membrane; constitutive secretory pathway; protein secretion; regulation of short-term neuronal synaptic plasticity; post-embryonic development; respiratory system process; synaptic transmission; intracellular protein transport; synaptic vesicle exocytosis; axonogenesis; glutamate secretion; sensory perception of touch; positive regulation of ATPase activity; neuromuscular synaptic transmission; maintenance of presynaptic active zone structure; Rab protein signal transduction; response to electrical stimulus; vesicle docking during exocytosis; positive regulation of exocytosis; lung development