Immunohistochemistry (IHC)
(Anti-MARCH4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Rabbit RNF174 / MARCH4 Polyclonal Antibody | anti-RNF174 antibody
Rabbit Polyclonal to Human RNF174 / MARCH4
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Immunohistochemistry (IHC)
(Anti-MARCH4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-MARCH4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB)
(Western blot of human RNF174 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at 0.5 ug/ml.)
Western Blot (WB)
(Western blot of human RNF174 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at 0.5 ug/ml.)
NCBI and Uniprot Product Information
NCBI Description
MARCH4 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH4 reduces surface accumulation of several membrane glycoproteins by directing them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]
Uniprot Description
MARCH4: E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Membrane protein, multi-pass; Ubiquitin ligase; EC 6.3.2.-; Ligase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: Golgi membrane; Golgi stack; integral to membrane; trans-Golgi network
Molecular Function: zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: protein ubiquitination
Research Articles on RNF174
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Product Notes
The RNF174 march4 (Catalog #AAA241687) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human RNF174 / MARCH4 reacts with Chimpanzee, Chicken, Dog, Horse, Human, Mouse, Opossum, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's RNF174 / MARCH4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB). IHC-P (5 ug/ml), WB (0.5 - 2 ug/ml) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the RNF174 march4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF174 / MARCH4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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