Rabbit EMI2 / FBXO43 Polyclonal Antibody | anti-EMI2 antibody

Rabbit Polyclonal to Human EMI2 / FBXO43

Cat. Num. AAA240573

• Gene Names: FBXO43; EMI2; ERP1; FBX43
• Reactivity: Chimpanzee, Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: EMI2 / FBXO43; Polyclonal Antibody; Rabbit Polyclonal to Human EMI2 / FBXO43; Anti-EMI2 / FBXO43 Antibody (N-Terminus) IHC-plus; FBXO43; Early mitotic inhibitor 2; EMI2; Endogenous meiotic inhibitor 2; F-box protein 43; ERP1; F-box only protein 43; FBX43; Human EMI2; anti-EMI2 antibody

• Host: Rabbit
• Reactivity: Chimpanzee, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: A region approximately 60 residues downstream of the amino terminal end of human Fbp5B protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 708

• Applicable Applications for anti-EMI2 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000 - 1:50000), IF, IHC-P (2.5 ug/ml), WB (1:500 - 1:3000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human FBXO43 / EMI2.
• Immunogen Type: Synthetic peptide
• Immunogen: EMI2 / FBXO43 antibody was raised against synthetic peptide from human FBXO43 / EMI2.
• Antigen Modification: N-Terminus
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-FBXO43 / EMI2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-Fbp5B Antibody - Western Blot. Western blot of affinity purified anti-Fbp5B antibody. Panels A and B show two identical membranes containing marker, in vitro translated Fbp5b (Fbp5b ivt), and a whole cell extract from 293T cells loaded in duplicate). Panel A shows antibody reactivity. Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide. This pre-incubation greatly diminishes specific band recognition. Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells. When this experiment was repeated using a mock in vitro translation, no band was detected (data not shown). Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute.)

Product Categories/Family for anti-EMI2 antibody

Family: F-Box

NCBI and Uniprot Product Information

• NCBI GI #: 117606351
• NCBI GeneID: 286151
• NCBI Accession #: NP_001025031.2
• NCBI GenBank Nucleotide #: NM_001029860.3
• UniProt Accession #: Q4G163
• Molecular Weight: 78,402 Da
• NCBI Official Full Name: F-box only protein 43
• NCBI Official Synonym Full Names: F-box protein 43
• NCBI Official Symbol: FBXO43
• NCBI Official Synonym Symbols: EMI2; ERP1; FBX43
• NCBI Protein Information: F-box only protein 43; early mitotic inhibitor 2; endogenous meiotic inhibitor 2
• UniProt Protein Name: F-box only protein 43
• Protein Family: Putative glucokinase
• UniProt Gene Name: FBXO43
• UniProt Synonym Gene Names: EMI2
• UniProt Entry Name: FBX43_HUMAN

NCBI Description

Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]

Uniprot Description

FBX43: Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Probably acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation (Probable). Part of a SCF (SKP1-cullin-F-box) protein ligase complex. May interact with CDC20.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: nucleus

Molecular Function: zinc ion binding

Biological Process: meiotic cell cycle; negative regulation of meiosis; protein ubiquitination; negative regulation of protein catabolic process