Rabbit anti-Human FN1 / Fibronectin Polyclonal Antibody | anti-FN1 antibody

Rabbit Polyclonal to Human FN1 / Fibronectin

Cat. Num. AAA240558

• Gene Names: FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: FN1 / Fibronectin; Polyclonal Antibody; Rabbit Polyclonal to Human FN1 / Fibronectin; Anti-FN1 / Fibronectin Antibody IHC-plus; FN1; CIG; Cold-insoluble globulin; Fibronectin 1; FINC; LETS; GFND; ED-B; Fibronectin; FN; FNZ; GFND2; Migration-stimulating factor; MSF; Human FN1; anti-FN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M K phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 2355

• Applicable Applications for anti-FN1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: ELISA (1:5000 - 1:10000), IHC-P (1.25 ug/ml), IP (1:100), WB (1:5000 - 1:10000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Preparation and Storage: Store vial at 4 degree C prior to opening. This product is stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20 C or below.

Immunohistochemistry (IHC)

(Anti-FN1 / Fibronectin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 16933542
• NCBI GeneID: 2335
• NCBI Accession #: NP_002017.1
• NCBI GenBank Nucleotide #: NM_002026.2
• UniProt Accession #: P02751; O95609; O95610; Q14312; Q14325; Q14326; Q17RV7; Q564H7; Q585T2; B7ZLF0; E9PE77; E9PG29
• Molecular Weight: 256,502 Da
• NCBI Official Full Name: fibronectin isoform 3 preproprotein
• NCBI Official Synonym Full Names: fibronectin 1
• NCBI Official Symbol: FN1
• NCBI Official Synonym Symbols: FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
• NCBI Protein Information: fibronectin; cold-insoluble globulin; migration-stimulating factor
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin
• UniProt Gene Name: FN1
• UniProt Synonym Gene Names: FN; FN; CIG
• UniProt Entry Name: FINC_HUMAN

NCBI Description

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Cell adhesion; Secreted

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: extracellular matrix; extracellular space; fibrinogen complex; apical plasma membrane; ER-Golgi intermediate compartment; extracellular region; basal lamina

Molecular Function: collagen binding; heparin binding; integrin binding; protein binding; protease activator activity; protease binding

Biological Process: integrin activation; platelet activation; extracellular matrix organization and biogenesis; positive regulation of axon extension; regulation of cell shape; extracellular matrix disassembly; platelet degranulation; acute-phase response; calcium-independent cell-matrix adhesion; cell-substrate junction assembly; response to wounding; peptide cross-linking; angiogenesis; blood coagulation; cell adhesion; leukocyte migration

Disease: Glomerulopathy With Fibronectin Deposits 2; Plasma Fibronectin Deficiency