Human Fibronectin ELISA Kit | FN1 elisa kit

Human Fibronectin ELISA Kit

Cat. Num. AAA289266

• Gene Names: FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
• Reactivity: Human
• Synonyms: Fibronectin; Human Fibronectin ELISA Kit; FN; Cold-insoluble globulin; CIG; FN1; FN1 elisa kit

• Reactivity: Human
• Sequence Length: 2446

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FN1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 807066342
• NCBI GeneID: 2335
• NCBI Accession #: NP_001293058.1
• NCBI GenBank Nucleotide #: NM_001306129.1
• UniProt Accession #: P02751; O95609; O95610; Q14312; Q14325; Q14326; Q17RV7; Q564H7; Q585T2; B7ZLF0; E9PE77; E9PG29
• Molecular Weight: 256,502 Da
• NCBI Official Full Name: fibronectin isoform 8
• NCBI Official Synonym Full Names: fibronectin 1
• NCBI Official Symbol: FN1
• NCBI Official Synonym Symbols: FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
• NCBI Protein Information: fibronectin
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin
• UniProt Gene Name: FN1
• UniProt Synonym Gene Names: FN; FN; CIG
• UniProt Entry Name: FINC_HUMAN

NCBI Description

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]

Uniprot Description

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.