Goat anti-Human POLK Polyclonal Antibody | anti-POLK antibody

Goat Polyclonal to Human POLK

Cat. Num. AAA240532

• Gene Names: POLK; DINP; POLQ; DINB1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: POLK; Polyclonal Antibody; Goat Polyclonal to Human POLK; Anti-POLK Antibody (aa817-830) IHC-plus; DINP; DNA polymerase kappa; DINB1; DINB protein; Human POLK; anti-POLK antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 870

• Applicable Applications for anti-POLK antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:2000 - 1:10000), IF, IHC-P (5 ug/ml), WB (1:500 - 1:3000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human POLK.
• Immunogen Type: Synthetic peptide
• Immunogen: POLK antibody was raised against synthetic peptide from human POLK.
• Antigen Modification: aa817-830
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-POLK antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-POLK Antibody - Western Blot. Western blot of Affinity Purified anti-POLK antibody shows detection of a band ~98 kD corresponding to human POLK (arrowhead). Approximately 35 ug of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred overnight at 4? followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)

NCBI and Uniprot Product Information

• NCBI GI #: 7705344
• NCBI GeneID: 51426
• NCBI Accession #: NP_057302.1
• NCBI GenBank Nucleotide #: NM_016218.2
• UniProt Accession #: Q9UBT6; Q5Q9G5; Q5Q9G6; Q5Q9G7; Q5Q9G8; Q86VJ8; Q8IZY0; Q8IZY1; Q8NB30; Q96L01; Q96Q86; B2RBD2
• Molecular Weight: 88,342 Da
• NCBI Official Full Name: DNA polymerase kappa
• NCBI Official Synonym Full Names: polymerase (DNA directed) kappa
• NCBI Official Symbol: POLK
• NCBI Official Synonym Symbols: DINP; POLQ; DINB1
• NCBI Protein Information: DNA polymerase kappa
• UniProt Protein Name: DNA polymerase kappa
• UniProt Gene Name: POLK
• UniProt Synonym Gene Names: DINB1; DINP
• UniProt Entry Name: POLK_HUMAN

NCBI Description

External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass (summary by Lone et al., 2007 [PubMed 17317631]).[supplied by OMIM, Jan 2010]

Uniprot Description

POLK: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Belongs to the DNA polymerase type-Y family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; Transferase; EC 2.7.7.7; DNA replication

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: nucleoplasm

Molecular Function: metal ion binding; damaged DNA binding; DNA-directed DNA polymerase activity

Biological Process: nucleotide-excision repair, DNA gap filling; DNA repair; DNA replication