Rabbit Catalase Polyclonal Antibody | anti-Y385 antibody

Catalase (Phospho-Y385) Polyclonal Antibody

Cat. Num. AAA3004969

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Catalase; Polyclonal Antibody; Catalase (Phospho-Y385) Polyclonal Antibody; CAT; anti-Y385 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-Catalase (Y385) polyclonal antibody detects endogenous levels of Catalase protein only when phosphorylated at Tyr385
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 527

• Applicable Applications for anti-Y385 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human Catalase around the phosphorylation site of Tyrosine 385.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-Catalase (Y385) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-Y385 antibody

Catalase is a peroxisome specific marker protein belonging to the catalase family. Defects in the gene encoding for the catalase protein can cause acatalasia, a disease characterized by the absence of catalase activity in red cells and associated with ulcerating oral lesions. Catalase is also an important regulator of oxidative stress and inflammation, and may contribute to the development of rheumatoid arthritis. Catalase, which can form a homotetramer, is found in all nearly all aerobically respiring organisms and functions in protecting cells from the toxic effects of hydrogen peroxide.

NCBI and Uniprot Product Information

• NCBI GI #: 4557014
• NCBI GeneID: 847
• NCBI Accession #: NP_001743.1
• NCBI GenBank Nucleotide #: NP_001743.1
• UniProt Accession #: P04040
• Molecular Weight: ~ 60kDa
• NCBI Official Full Name: catalase
• NCBI Official Synonym Full Names: catalase
• NCBI Official Symbol: CAT
• NCBI Protein Information: catalase
• UniProt Protein Name: Catalase
• Protein Family: Catalase
• UniProt Gene Name: CAT
• UniProt Entry Name: CATA_HUMAN

NCBI Description

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

Catalase: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. Homotetramer. Belongs to the catalase family.

Protein type: Amino Acid Metabolism - tryptophan; Endoplasmic reticulum; EC 1.11.1.6; Apoptosis; Energy Metabolism - methane; Mitochondrial; Hydrolase; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: peroxisomal membrane; Golgi apparatus; peroxisomal matrix; focal adhesion; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; lysosome; plasma membrane; mitochondrial intermembrane space; peroxisome; cytosol

Molecular Function: antioxidant activity; oxidoreductase activity, acting on peroxide as acceptor; enzyme binding; protein homodimerization activity; catalase activity; metal ion binding; heme binding; NADP binding; aminoacylase activity; receptor binding

Biological Process: cholesterol metabolic process; hemoglobin metabolic process; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; activation of NF-kappaB transcription factor; protein homotetramerization; osteoblast differentiation; positive regulation of phosphoinositide 3-kinase cascade; response to vitamin E; response to reactive oxygen species; triacylglycerol metabolic process; response to hyperoxia; UV protection; hydrogen peroxide catabolic process; inhibition of NF-kappaB transcription factor; ureteric bud development; positive regulation of cell division; response to hypoxia; aerobic respiration; purine nucleotide catabolic process; protein tetramerization; negative regulation of apoptosis; aging

Disease: Acatalasemia