Immunohistochemistry (IHC)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit anti-Human RCHY1/PIRH2 Polyclonal Antibody | anti-RCHY1/PIRH2 antibody
Anti-RCHY1/PIRH2 Antibody
Gene Names
RCHY1; ZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
RCHY1/PIRH2; Polyclonal Antibody; Anti-RCHY1/PIRH2 Antibody; Mouse Monoclonal [clone 1H10] (IgG1) to Human RCHY1/PIRH2; RCHY1; ARNIP; CHIMP; HARNIP; HPirh2; PIRH2E; PIRH2; PIRH2F; PRO1996; RING finger protein 199; Zinc finger protein 363; ZNF363; p53-induced RING-H2 protein; RNF199; anti-RCHY1/PIRH2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human RCHY1/PIRH2
Purity/Purification
Immunoaffinity Purified
Form/Format
0.1 M Tris-glycine, pH 7, 20% Glycerol, 0.01% Thimerosal
Sequence Length
261
Applicable Applications for anti-RCHY1/PIRH2 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
ICC: 1:100-1:1000
IF: 1:100-1:1000
IHC-P: 5 ug/ml
WB: 1:500-1:3000
IF: 1:100-1:1000
IHC-P: 5 ug/ml
WB: 1:500-1:3000
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 59 and 261 (Q96PM5) of Pirh2. Percent identity by BLAST analysis: Human (100%); Rat (94%); Mouse (93%).
Conjugation
Unconjugated
Preparation and Storage
Aliquot and freeze at -20 degree C. Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF)
(Immunofluorescence of paraformaldehyde-fixed HeLa, using Pirh2 antibody at 1:500 dilution.)
Immunofluorescence (IF)
(Immunofluorescence of paraformaldehyde-fixed HeLa, using Pirh2 antibody at 1:500 dilution.)
Western Blot (WB)
(Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE RCHY1/PIRH2 antibody diluted at 1:1000)
Western Blot (WB)
(Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE RCHY1/PIRH2 antibody diluted at 1:1000)
Related Product Information for anti-RCHY1/PIRH2 antibody
Mediates E3-dependent ubiquitination and proteasomal degradation oftarget proteins, including p53/TP53, P73, HDAC1 and CDKN1B.Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,210 Da
NCBI Official Full Name
RING finger and CHY zinc finger domain-containing protein 1 isoform 1
NCBI Official Synonym Full Names
ring finger and CHY zinc finger domain containing 1
NCBI Official Symbol
RCHY1
NCBI Official Synonym Symbols
ZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996
NCBI Protein Information
RING finger and CHY zinc finger domain-containing protein 1
UniProt Protein Name
RING finger and CHY zinc finger domain-containing protein 1
UniProt Gene Name
RCHY1
UniProt Synonym Gene Names
ARNIP; CHIMP; PIRH2; RNF199; ZNF363; hPirh2
NCBI Description
The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2013]
Uniprot Description
Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.
Research Articles on RCHY1/PIRH2
Similar Products
Product Notes
The RCHY1/PIRH2 rchy1 (Catalog #AAA2401665) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RCHY1/PIRH2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RCHY1/PIRH2 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ICC: 1:100-1:1000 IF: 1:100-1:1000 IHC-P: 5 ug/ml WB: 1:500-1:3000. Researchers should empirically determine the suitability of the RCHY1/PIRH2 rchy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RCHY1/PIRH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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