Rabbit ID4 Polyclonal Antibody | anti-ID4 antibody

Anti-ID4 Antibody (aa1-50) IHC-plus

Cat. Num. AAA2400314

• Gene Names: ID4; IDB4; bHLHb27
• Reactivity: Mouse, Rat, Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity purified
• Synonyms: ID4; Polyclonal Antibody; Anti-ID4 Antibody (aa1-50) IHC-plus; Rabbit Polyclonal (IgG) to Human ID4; Human ID4; BHLHb27; IDB4; Inhibitor of DNA binding 4; anti-ID4 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ID4 Antibody detects endogenous levels of total ID4 protein.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 161

• Applicable Applications for anti-ID4 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:20000); IHC-P (10 ug/ml); WB (1:500 - 1:1000)
• Immunogen: ID4 antibody was raised against synthetic peptide from human ID4 (aa1-50).
• Immunogen Description: Synthetic peptide from human ID4 (aa1-50).
• Antigen Modification: aa1-50
• Target Species: Human
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-ID4 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts from HepG2 cells, using ID4 Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-ID4 antibody

ID4 Antibody, BHLHb27 Antibody, IDB4 Antibody, Inhibitor of DNA binding 4 Antibody Description: Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation.

NCBI and Uniprot Product Information

• NCBI GI #: 4504573
• NCBI GeneID: 3400
• NCBI Accession #: NP_001537.1
• NCBI GenBank Nucleotide #: NM_001546.3
• UniProt Accession #: P47928; Q13005
• Molecular Weight: 16,622 Da
• NCBI Official Full Name: DNA-binding protein inhibitor ID-4
• NCBI Official Synonym Full Names: inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
• NCBI Official Symbol: ID4
• NCBI Official Synonym Symbols: IDB4; bHLHb27
• NCBI Protein Information: DNA-binding protein inhibitor ID-4
• UniProt Protein Name: DNA-binding protein inhibitor ID-4
• Protein Family: DNA-binding protein inhibitor
• UniProt Gene Name: ID4
• UniProt Synonym Gene Names: BHLHB27; bHLHb27
• UniProt Entry Name: ID4_HUMAN

NCBI Description

This gene encodes a member of the inhibitor of DNA binding (ID) protein family. These proteins are basic helix-loop-helix transcription factors which can act as tumor suppressors but lack DNA binding activity. Consequently, the activity of the encoded protein depends on the protein binding partner. [provided by RefSeq, Dec 2011]

Uniprot Description

ID4: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; protein dimerization activity; transcription corepressor activity

Biological Process: cerebral cortex neuron differentiation; circadian rhythm; fat cell differentiation; G1/S transition of mitotic cell cycle; hippocampus development; myelination in the central nervous system; negative regulation of astrocyte differentiation; negative regulation of fat cell differentiation; negative regulation of neuron differentiation; negative regulation of oligodendrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neuroblast proliferation; positive regulation of cell proliferation; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent