Mouse ID4 Monoclonal Antibody | anti-ID4 antibody

ID4 (Inhibitor of DNA Binding 4, Dominant Negative Helix-loop-Helix Protein, IDB4, bHLHb27) (FITC)

Cat. Num. AAA6178696

• Gene Names: ID4; IDB4; bHLHb27
• Applications: Western Blot
• Purity: Purified
• Synonyms: ID4; Monoclonal Antibody; ID4 (Inhibitor of DNA Binding 4; Dominant Negative Helix-loop-Helix Protein; IDB4; bHLHb27) (FITC); Inhibitor of DNA Binding 4; bHLHb27; anti-ID4 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3F11
• Specificity: Recognizes ID4.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-ID4 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: ID4 (NP_001537.1, 1aa-161aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; ; FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged ID4 is 1 ng/ml as a capture antibody.)

Western Blot (WB)

(Western Blot analysis of ID4 expression in transfected 293T cell line by ID4 monoclonal antibody (M13), clone 3F11.Lane 1: ID4 transfected lysate (Predicted MW: 16.6 KDa).Lane 2: Non-transfected lysate.)

Related Product Information for anti-ID4 antibody

Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 [PubMed 7665172]). [supplied by OMIM]

Product Categories/Family for anti-ID4 antibody

Antibodies; Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4504573
• NCBI GeneID: 3400
• NCBI Accession #: NP_001537.1
• NCBI GenBank Nucleotide #: NM_001546.3
• UniProt Accession #: P47928; Q13005
• Molecular Weight: 16,622 Da
• NCBI Official Full Name: DNA-binding protein inhibitor ID-4
• NCBI Official Synonym Full Names: inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
• NCBI Official Symbol: ID4
• NCBI Official Synonym Symbols: IDB4; bHLHb27
• NCBI Protein Information: DNA-binding protein inhibitor ID-4; class B basic helix-loop-helix protein 27
• UniProt Protein Name: DNA-binding protein inhibitor ID-4
• Protein Family: DNA-binding protein inhibitor
• UniProt Gene Name: ID4
• UniProt Synonym Gene Names: BHLHB27; bHLHb27
• UniProt Entry Name: ID4_HUMAN

NCBI Description

This gene encodes a member of the inhibitor of DNA binding (ID) protein family. These proteins are basic helix-loop-helix transcription factors which can act as tumor suppressors but lack DNA binding activity. Consequently, the activity of the encoded protein depends on the protein binding partner. [provided by RefSeq, Dec 2011]

Uniprot Description

ID4: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; protein binding; transcription corepressor activity

Biological Process: fat cell differentiation; circadian rhythm; transcription, DNA-dependent; myelination in the central nervous system; hippocampus development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; cerebral cortex neuron differentiation; regulation of transcription from RNA polymerase II promoter; negative regulation of oligodendrocyte differentiation; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; positive regulation of cell proliferation; negative regulation of astrocyte differentiation; neuroblast proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle