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Immunohistochemistry (IHC) (Anti-DCTN1 / Dynactin 1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Rabbit anti-Human DCTN1 / Dynactin 1 Polyclonal Antibody | anti-DCTN1 antibody

Anti-DCTN1 / Dynactin 1 Antibody (aa1216-1278) IHC-plus

Gene Names
DCTN1; P135; DP-150; DAP-150
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Immunoaffinity purified
Synonyms
DCTN1 / Dynactin 1; Polyclonal Antibody; Anti-DCTN1 / Dynactin 1 Antibody (aa1216-1278) IHC-plus; Rabbit Polyclonal (IgG) to Human DCTN1 / Dynactin 1; Human DCTN1 / Dynactin 1; DAP-150; Dynactin subunit 1; DP-150; Dynactin 1; HMN7B; p150-glued; p135; anti-DCTN1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human DCTN1
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, pH 7.0, 1% BSA, 20% glycerol, 0.01% Thimerosal
Concentration
1 mg/ml (varies by lot)
Sequence Length
1144
Applicable Applications for anti-DCTN1 antibody
Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
ICC (1:100 - 1:1000)
IHC-P (5 ug/ml)
WB (1:500 - 1:3000)
Immunogen
DCTN1 / Dynactin 1 antibody was raised against synthetic peptide corresponding to a region within amino acids 1216 and 1278 of DCTN1 (SwissProt Q14203). Percent identity by BLAST analysis: Human, Mouse (100%).
Immunogen Description
Synthetic peptide corresponding to a region within amino acids 1216 and 1278 of DCTN1 (SwissProt Q14203). Percent identity by BLAST analysis: Human, Mouse (100%).
Immunogen Type
Synthetic peptide
Antigen Modification
aa1216-1278
Target Species
Human
Preparation and Storage
Keep as concentrated solution. Aliquot and store at -20 degree C or below. Avoid multiple freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-DCTN1 / Dynactin 1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-DCTN1 / Dynactin 1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunocytochemistry (ICC)

(Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa using DCTN1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.)

Immunocytochemistry (ICC) (Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa using DCTN1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.)

Western Blot (WB)

(Sample (30 ug of whole cell lysate). A: JurKat. 5% SDS PAGE. DCTN1 antibody diluted at 1:3000.)

Western Blot (WB) (Sample (30 ug of whole cell lysate). A: JurKat. 5% SDS PAGE. DCTN1 antibody diluted at 1:3000.)
Related Product Information for anti-DCTN1 antibody
DCTN1 Antibody, DAP-150 Antibody, Dynactin subunit 1 Antibody, DP-150 Antibody, Dynactin 1 Antibody, HMN7B Antibody, p150-glued Antibody, p135 Antibody Description: DCTN1 / Dynactin 1 is the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
140,887 Da
NCBI Official Full Name
dynactin subunit 1 isoform 2
NCBI Official Synonym Full Names
dynactin subunit 1
NCBI Official Symbol
DCTN1
NCBI Official Synonym Symbols
P135; DP-150; DAP-150
NCBI Protein Information
dynactin subunit 1
UniProt Protein Name
Dynactin subunit 1
Protein Family
UniProt Gene Name
DCTN1
UniProt Synonym Gene Names
DP-150
UniProt Entry Name
DCTN1_HUMAN

NCBI Description

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]

Uniprot Description

dynactin 1: Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein- dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B); also known as progressive lower motor neuron disease (PLMND). HMN7B is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs. Defects in DCTN1 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS). ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. Defects in DCTN1 are the cause of Perry syndrome (PERRYS); also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally. Belongs to the dynactin 150 kDa subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: centrosome; cytoplasm; cytosol; dynactin complex; dynein complex; kinetochore; leading edge; membrane; microtubule; retromer complex; spindle pole

Molecular Function: dynein binding; motor activity; protein binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; cellular protein metabolic process; ER to Golgi vesicle-mediated transport; G2/M transition of mitotic cell cycle; melanosome transport; mitosis; mitotic cell cycle; nervous system development; organelle organization and biogenesis; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; retrograde transport, endosome to Golgi; unfolded protein response

Disease: Amyotrophic Lateral Sclerosis 1; Neuronopathy, Distal Hereditary Motor, Type Viib; Perry Syndrome

Research Articles on DCTN1

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Product Notes

The DCTN1 dctn1 (Catalog #AAA2400121) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DCTN1 / Dynactin 1 Antibody (aa1216-1278) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DCTN1 / Dynactin 1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB). ICC (1:100 - 1:1000) IHC-P (5 ug/ml) WB (1:500 - 1:3000). Researchers should empirically determine the suitability of the DCTN1 dctn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DCTN1 / Dynactin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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