Rabbit DCTN1 Polyclonal Antibody | anti-DCTN1 antibody

DCTN1 Antibody

Cat. Num. AAA2525518

• Gene Names: DCTN1; P135; DP-150; DAP-150
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: DCTN1; Polyclonal Antibody; DCTN1 Antibody; DAP-150; DP-150; HMN7B; P135; anti-DCTN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1271

• Applicable Applications for anti-DCTN1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Recombinant protein of human DCTN1
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-9803 / sc-9804 / sc-9801 / sc-11363
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using DCTN1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophageal cancer using DCTN1 antibody at dilution of 1:100 (200x lens).)

Related Product Information for anti-DCTN1 antibody

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]

NCBI and Uniprot Product Information

• NCBI GI #: 299890875
• NCBI GeneID: 1639
• NCBI Accession #: NP_001177766.1
• NCBI GenBank Nucleotide #: NM_001190837.1
• Molecular Weight: 140,887 Da
• NCBI Official Full Name: dynactin subunit 1 isoform 6
• NCBI Official Synonym Full Names: dynactin 1
• NCBI Official Symbol: DCTN1
• NCBI Official Synonym Symbols: P135; DP-150; DAP-150
• NCBI Protein Information: dynactin subunit 1; 150 kDa dynein-associated polypeptide; dynactin 1 (p150, glued homolog, Drosophila)
• UniProt Protein Name: Dynactin subunit 1
• Protein Family: Dynactin
• UniProt Gene Name: DCTN1
• UniProt Synonym Gene Names: DP-150
• UniProt Entry Name: DCTN1_HUMAN

NCBI Description

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]

Uniprot Description

dynactin 1: Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein- dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B); also known as progressive lower motor neuron disease (PLMND). HMN7B is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs. Defects in DCTN1 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS). ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. Defects in DCTN1 are the cause of Perry syndrome (PERRYS); also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally. Belongs to the dynactin 150 kDa subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis; Microtubule-binding

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: kinetochore; dynein complex; spindle pole; microtubule; centrosome; dynactin complex; membrane; retromer complex; cytoplasm; leading edge; cytosol

Molecular Function: dynein binding; protein binding; motor activity

Biological Process: mitosis; nervous system development; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; unfolded protein response; organelle organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; mitotic cell cycle; G2/M transition of mitotic cell cycle; retrograde transport, endosome to Golgi; melanosome transport

Disease: Amyotrophic Lateral Sclerosis 1; Perry Syndrome; Neuronopathy, Distal Hereditary Motor, Type Viib