IFN GAMMA Active Protein | Ifng active protein

RECOMBINANT MOUSE INTERFERON GAMMA

Cat. Num. AAA232344

• Gene Names: Ifng; Ifg; IFN-g
• Applications: ELISA, Functional Assay, Western Blot
• Purity: >98% by SDS PAGE and HPLC
• Synonyms: IFN GAMMA; RECOMBINANT MOUSE INTERFERON GAMMA; Ifng active protein

• Purity/Purification: >98% by SDS PAGE and HPLC
• Form/Format: Purified recombinant protein - lyophilised
• Concentration: Total protein concentration 1.0mg/ml after reconstitution (varies by lot)
• Sequence Length: 155

• Applicable Applications for Ifng active protein: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;; Functional Assays: Minimum Dilution: 0.1; Maximum Dilution: 1.0ng/ml;; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
• Reconstitution: Reconstitute with 20ul water. For extended storage the addition of 0.1% BSA is recommended. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.; Preparation
• Buffer Solution: Phosphate buffered saline
• Activity: >5 x 106 units/mg as determined by ability to inhibit the proliferation of murine WEHI-279 cells.
• Target Species: Mouse
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 3 months from date of reconstitution.

Related Product Information for Ifng active protein

Recombinant mouse interferon gamma is an E Coli-derived recombinant protein corresponding to full-length mature murine interferon gamma, a regulatory cytokine, which is produced by activated natural killer cells and certain T-lymphocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 33468859
• NCBI GeneID: 15978
• NCBI Accession #: NP_032363.1
• NCBI GenBank Nucleotide #: NM_008337.3
• UniProt Accession #: P01580; Q542B8
• Molecular Weight: Protein Molecular Weight: 15.6kD (134 amino acid sequence)
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon gamma
• NCBI Official Symbol: Ifng
• NCBI Official Synonym Symbols: Ifg; IFN-g
• NCBI Protein Information: interferon gamma; IFN-gamma; gamma interferon
• UniProt Protein Name: Interferon gamma
• Protein Family: Interferon
• UniProt Gene Name: Ifng
• UniProt Synonym Gene Names: IFN-gamma
• UniProt Entry Name: IFNG_MOUSE

NCBI Description

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]

Uniprot Description

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Secreted, signal peptide; Secreted; Membrane protein, integral; Cytokine

Cellular Component: extracellular space; cell; extracellular region; intracellular; external side of plasma membrane

Molecular Function: protein binding; interferon-gamma receptor binding; cytokine activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of cell adhesion; positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; positive regulation of killing of cells of another organism; positive regulation of membrane protein ectodomain proteolysis; regulation of growth; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; neutrophil chemotaxis; positive regulation of synaptic transmission, cholinergic; CD8-positive, alpha-beta T cell differentiation during immune response; negative regulation of myelination; response to virus; positive regulation of tumor necrosis factor production; defense response to protozoan; inflammatory cell apoptosis; positive regulation of tyrosine phosphorylation of Stat1 protein; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; apoptosis; positive regulation of interleukin-12 production; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-6 biosynthetic process; sensory perception of mechanical stimulus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; adaptive immune response; regulation of immune response; unfolded protein response; humoral immune response; antigen processing and presentation; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; immune response; positive regulation of neuron differentiation; regulation of insulin secretion