ERYTHROPOIETIN ALPHA Active Protein | EPO active protein

RECOMBINANT HUMAN ERYTHROPOIETIN ALPHA

Cat. Num. AAA232058

• Gene Names: EPO; EP; MVCD2
• Applications: Functional Assay
• Purity: >98.0% by HPLC analysis and SDS-PAGE
• Synonyms: ERYTHROPOIETIN ALPHA; RECOMBINANT HUMAN ERYTHROPOIETIN ALPHA; EPO active protein

• Purity/Purification: >98.0% by HPLC analysis and SDS-PAGE
• Form/Format: Recombinant protein - lyophilised
• Concentration: Total protein concentration 0.2mg/ml after reconstitution (varies by lot)
• Sequence Length: 193

• Applicable Applications for EPO active protein: Functional Assays (FN)
• Reconstitution: Reconstitute with 250ul sterile deionised water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of a carrier protein (0.1% HSA or BSA) is recommended.
• Buffer Solution: Citrate buffered saline
• Activity: The specific activity was measured by normocythaemic mice and found to be 150,000 IU/mg
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Related Product Information for EPO active protein

MBS232058 is recombinant human erythropoietein alpha, produced in CHO cells. It is a single polypeptide chain containing 166 amino acids with a predicted molecular mass of 21,000 Daltons and an apparent molecular mass of 30,400 Daltons.

NCBI and Uniprot Product Information

• NCBI GI #: 62240997
• NCBI GeneID: 2056
• NCBI Accession #: NP_000790.2
• NCBI GenBank Nucleotide #: NM_000799.2
• UniProt Accession #: P01588; Q2M2L6; Q549U2; Q9UDZ0; Q9UEZ5; Q9UHA0
• Molecular Weight: 21,307 Da
• NCBI Official Full Name: erythropoietin
• NCBI Official Synonym Full Names: erythropoietin
• NCBI Official Symbol: EPO
• NCBI Official Synonym Symbols: EP; MVCD2
• NCBI Protein Information: erythropoietin; epoetin
• UniProt Protein Name: Erythropoietin
• Protein Family: Erythropoietin
• UniProt Gene Name: EPO
• UniProt Entry Name: EPO_HUMAN

NCBI Description

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008]

Uniprot Description

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: protein binding; erythropoietin receptor binding; hormone activity; protein kinase activator activity

Biological Process: hemoglobin biosynthetic process; erythrocyte maturation; blood circulation; apoptosis; positive regulation of transcription, DNA-dependent; response to lipopolysaccharide; response to testosterone stimulus; signal transduction; response to salt stress; response to vitamin A; peptidyl-serine phosphorylation; response to hyperoxia; positive regulation of tyrosine phosphorylation of Stat5 protein; response to estrogen stimulus; positive regulation of protein kinase activity; positive regulation of cell proliferation; positive regulation of Ras protein signal transduction; response to axon injury; positive regulation of neuron differentiation; response to electrical stimulus; positive regulation of DNA replication; embryo implantation; aging

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 2