Testing Data
(FigureA. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse anti Human CD4 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Mouse anti-Human CD4 Monoclonal Antibody | anti-CD4 antibody
Mouse Anti Human CD4: Amethyst Orange
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
This product should be stored undiluted.
Storage in frost free freezers is not recommended.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
This product contains a precipitate we recommend microcentrifugation before use.
Testing Data
(FigureA. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse anti Human CD4 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(FigureA. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse anti Human CD4 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD3 and Alexa Fluor 700 conjugated mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD3 and Alexa Fluor 700 conjugated Mouse anti Human CD4 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD3 and Alexa Fluor 700 conjugated mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD3 and Alexa Fluor 700 conjugated Mouse anti Human CD4 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse anti Human CD4 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Mouse anti Human CD4 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 and RPE conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 and RPE conjugated Mouse anti Human CD4 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 and RPE conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 and RPE conjugated Mouse anti Human CD4 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. FITC conjugated mouse anti human CD3 and Alexa 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD3 and Alexa 647 conjugated mouse anti human CD4 . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Testing Data
(Figure A. FITC conjugated mouse anti human CD3 and Alexa 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD3 and Alexa 647 conjugated mouse anti human CD4 . All experiments performed on red cell lysed human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Testing Data
(Figure A. FITC conjugated mouse anti human CD3 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD3 and RPE conjugated mouse anti human CD4 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Testing Data
(Figure A. FITC conjugated mouse anti human CD3 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD3 and RPE conjugated mouse anti human CD4 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Testing Data
(Figure A. RPE conjugated mouse anti human CD3 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti human CD3 and FITC conjugated mouse anti human CD4 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Testing Data
(Figure A. RPE conjugated mouse anti human CD3 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti human CD3 and FITC conjugated mouse anti human CD4 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Testing Data
(Figure A. Pacific Blue conjugated mouse anti human CD19 and Alexa488 conjugated Mouse IgG1 isotype control . Figure B. Pacific Blue conjugated mouse anti human CD19 and Alexa488 conjugated mouse anti human CD4 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Testing Data
(Figure A. Pacific Blue conjugated mouse anti human CD19 and Alexa488 conjugated Mouse IgG1 isotype control . Figure B. Pacific Blue conjugated mouse anti human CD19 and Alexa488 conjugated mouse anti human CD4 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock .)
Testing Data
(Figure A. RPE conjugated Rat anti Human CD28 and A647 conjugated Mouse IgG1 istoype control . Figure B. RPE conjugated Rat anti Human CD28 and A647 conjugated Mouse anti Human CD4 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)
Testing Data
(Figure A. RPE conjugated Rat anti Human CD28 and A647 conjugated Mouse IgG1 istoype control . Figure B. RPE conjugated Rat anti Human CD28 and A647 conjugated Mouse anti Human CD4 . All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Low power.)
Testing Data
(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Low power.)
Mouse anti human CD4 antibody, clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation (Piatier-Tonneau et al, 1997). Recommend the use of Mouse anti Human CD4:Low Endotoxin (MCA1267EL) for this purpose.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, May 2020]
Uniprot Description
CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.
Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; integral to membrane; plasma membrane; external side of plasma membrane; lipid raft
Molecular Function: protein binding; transmembrane receptor activity; protein homodimerization activity; enzyme binding; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding
Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; induction by virus of cell-cell fusion in host; defense response to Gram-negative bacterium; signal transduction; T cell receptor signaling pathway; enzyme linked receptor protein signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; T cell costimulation; protein palmitoleylation; innate immune response; immune response; entry into host cell; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of T cell activation; T cell differentiation
Disease: Okt4 Epitope Deficiency
Research Articles on CD4
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Product Notes
The CD4 cd4 (Catalog #AAA225854) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD4: Amethyst Orange reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Min. Dilution: Neat Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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