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Testing Data (Figure A. Alexa Fluor 700 conjugated Rat anti Mouse CD3 and FITC conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 700 conjugated Rat anti Mouse CD3 and FITC conjugated Rat anti Mouse CD4 . All expe)

Rat anti-Mouse CD4 Monoclonal Antibody | anti-CD4 antibody

Rat anti Mouse CD4:Amethyst Orange

Gene Names
Cd4; L3T4; Ly-4
Reactivity
Mouse
Applications
ELISA
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
CD4; Monoclonal Antibody; Rat anti Mouse CD4:Amethyst Orange; CD4 antibody | RM4-5; anti-CD4 antibody
Ordering
For Research Use Only!
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
RM4-5
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Amethyst Orange - liquid
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Applicable Applications for anti-CD4 antibody
ELISA (EIA)
Application Notes
FC/FACS: Neat
Immunogen
BALB/c mouse thymocytes
Conjugation
Amethyst Orange
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Testing Data

(Figure A. Alexa Fluor 700 conjugated Rat anti Mouse CD3 and FITC conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 700 conjugated Rat anti Mouse CD3 and FITC conjugated Rat anti Mouse CD4 . All expe)

Testing Data (Figure A. Alexa Fluor 700 conjugated Rat anti Mouse CD3 and FITC conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 700 conjugated Rat anti Mouse CD3 and FITC conjugated Rat anti Mouse CD4 . All expe)

Testing Data

(Figure A. Pacific Blue conjugated Rat anti Mouse CD3 and RPE conjugated Rat IgG2a isotype control . Figure B. Pacific Blue conjugated Rat anti Mouse CD3 and RPE conjugated Rat anti Mouse CD4 . All experiments pe)

Testing Data (Figure A. Pacific Blue conjugated Rat anti Mouse CD3 and RPE conjugated Rat IgG2a isotype control . Figure B. Pacific Blue conjugated Rat anti Mouse CD3 and RPE conjugated Rat anti Mouse CD4 . All experiments pe)

Testing Data

(Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD3 and Alexa Fluor 647 conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Rat anti Mou)

Testing Data (Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD3 and Alexa Fluor 647 conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Rat anti Mou)

Testing Data

(Figure A. RPE conjugated Rat anti Mouse CD3 and Alexa Fluor 488 conjugated Rat IgG2a isotype control . Figure B. PE conjugated Rat anti Mouse CD3 and Alexa Fluor 488 conjugated Rat anti Mouse CD4 . All exper)

Testing Data (Figure A. RPE conjugated Rat anti Mouse CD3 and Alexa Fluor 488 conjugated Rat IgG2a isotype control . Figure B. PE conjugated Rat anti Mouse CD3 and Alexa Fluor 488 conjugated Rat anti Mouse CD4 . All exper)

Testing Data

(Figure A. RPE conjugated Rat anti Mouse CD3 and Alexa Fluor 647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD3 and Alexa Fluor 647 conjugated Rat anti Mouse CD4 . All expe)

Testing Data (Figure A. RPE conjugated Rat anti Mouse CD3 and Alexa Fluor 647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD3 and Alexa Fluor 647 conjugated Rat anti Mouse CD4 . All expe)

Testing Data

(Figure A. Alexa Fluor 647 conjugated Rat anti Mouse CD3 and FITC conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Rat anti Mouse CD3 and FITC conjugated Rat anti Mouse CD4 . All expe)

Testing Data (Figure A. Alexa Fluor 647 conjugated Rat anti Mouse CD3 and FITC conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Rat anti Mouse CD3 and FITC conjugated Rat anti Mouse CD4 . All expe)

Testing Data

(Figure A. FITC conjugated Rat anti Mouse CD4 . Figure B. FITC conjugated Rat anti Mouse CD4 and SBV515 conjugated Rat anti Mouse CD3 . All experiments performed on red cell lysed mouse blood gated on live single cell lym)

Testing Data (Figure A. FITC conjugated Rat anti Mouse CD4 . Figure B. FITC conjugated Rat anti Mouse CD4 and SBV515 conjugated Rat anti Mouse CD3 . All experiments performed on red cell lysed mouse blood gated on live single cell lym)

Testing Data

(Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD3 . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD3 and SBV515 conjugated Rat anti Mouse CD4 . All experiments performed on red cell lysed mouse blood ga)

Testing Data (Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD3 . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD3 and SBV515 conjugated Rat anti Mouse CD4 . All experiments performed on red cell lysed mouse blood ga)

Testing Data

(Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD3 . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD3 and SBV440 conjugated Rat anti Mouse CD4 . All experiments performed on mouse blood gated on live sin)

Testing Data (Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD3 . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD3 and SBV440 conjugated Rat anti Mouse CD4 . All experiments performed on mouse blood gated on live sin)

Testing Data

(Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD4 . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD4 and StarBright Violet 610 conjugated Rat anti Mouse CD3 . All experiments performed on red blood lys)

Testing Data (Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD4 . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD4 and StarBright Violet 610 conjugated Rat anti Mouse CD3 . All experiments performed on red blood lys)
Related Product Information for anti-CD4 antibody
Rat anti Mouse CD4 antibody, clone RM4-5 detects mouse CD4, a 55kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance.

Rat anti Mouse CD4 antibody, clone RM4-5 can be used for in vitro blocking of ligand binding, as well as in vitro CD4+ T cell depletions.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,473 Da
NCBI Official Full Name
T-cell surface glycoprotein CD4
NCBI Official Synonym Full Names
CD4 antigen
NCBI Official Symbol
Cd4
NCBI Official Synonym Symbols
L3T4; Ly-4
NCBI Protein Information
T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)
UniProt Protein Name
T-cell surface glycoprotein CD4
Protein Family
UniProt Gene Name
Cd4
UniProt Entry Name
CD4_MOUSE

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Product Notes

The CD4 cd4 (Catalog #AAA226116) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat anti Mouse CD4:Amethyst Orange reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). FC/FACS: Neat. Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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