Rabbit anti-Human PKA C-ALPHA Polyclonal Antibody | anti-PRKACA antibody

Rabbit Anti PKA C-ALPHA

Cat. Num. AAA225828

• Gene Names: PRKACA; PKACA; PPNAD4
• Reactivity: Human
• Applications: Western Blot
• Purity: Antiserum to PKA C-alpha was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Synonyms: PKA C-ALPHA; Polyclonal Antibody; Rabbit Anti PKA C-ALPHA; PKA C-ALPHA antibody; anti-PRKACA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antiserum to PKA C-alpha was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Form/Format: Purified IgG - Liquid
• Sequence Length: 351

• Applicable Applications for anti-PRKACA antibody: Western Blot (WB)
• Application Notes: WB: 1/500-2000
• Immunogen: A synthetic peptide corresponding to amino acids 9-40 of human PKA C-alpha
• Preservative Stabilizers: 0.02% Sodium Azide
• Buffer Solution: Phosphate Buffered Saline
• Preparation and Storage: Store at -20 degree C.; Storage in frost-free freezers is not recommended.; This product should be stored undiluted.; Avoid repeated freezing and thawing as this may denature the antibody.; This product contains a precipitate we recommend microcentrifugation before use.

Western Blot (WB)

(Western blot analysis of HeLa cell lysate using Rabbit anti PKA C-alpha antibody. 3% non-fat dry milk was used for blocking)

Related Product Information for anti-PRKACA antibody

Rabbit anti PKA C-alpha antibody recognizes cAMP-dependent protein kinase catalytic subunit alpha, also known as PRKACA. The function and specificity of PKA C-alpha depend on subcellular localization, expression of isoforms and physio-chemical features (Søberg et al. 2013, Skalhegg and Tasken 2000). It phosphorylates a large number of proteins, for example, p50 at serine 337 (Guan et al. 2005), claudin-4 at threonine 192 (D'Souza et al. 2005). Dnajb1-Prkaca gene fusion drives tumorigenesis in mice (Kastenhuber et al. 2017).

NCBI and Uniprot Product Information

• NCBI GI #: 4506055
• NCBI GeneID: 5566
• NCBI Accession #: NP_002721.1
• NCBI GenBank Nucleotide #: NP_002721.1
• UniProt Accession #: P17612
• NCBI Official Full Name: cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1
• NCBI Official Synonym Full Names: protein kinase cAMP-activated catalytic subunit alpha
• NCBI Official Symbol: PRKACA
• NCBI Official Synonym Symbols: PKACA; PPNAD4
• NCBI Protein Information: cAMP-dependent protein kinase catalytic subunit alpha
• UniProt Protein Name: cAMP-dependent protein kinase catalytic subunit alpha
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKACA
• UniProt Synonym Gene Names: PKACA; PKA C-alpha
• UniProt Entry Name: KAPCA_HUMAN

NCBI Description

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]