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Testing Data
(Figure A. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat IgG2a isotype control . Figure B. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat anti Canine CD5 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
IgG2a negative control
Rat IgG2a Negative Control
Reactivity
Rat
Applications
Flow Cytometry, Functional Assay
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
IgG2a; Rat IgG2a Negative Control; Rat IgG2a Negative Control antibody; IgG2a negative control
Host
Rat
Reactivity
Rat
Isotype
IgG2a
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Liquid; Phosphate Buffered Saline
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for IgG2a negative control
Flow Cytometry (FC/FACS)
Immunogen
Trinitrophenol
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Figure A. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat IgG2a isotype control . Figure B. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat anti Canine CD5 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat IgG2a isotype control . Figure B. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat anti Canine CD5 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. FITC conjugated Mouse anti Canine CD3 and purified Rat IgG2a isotype control detected with Goat anti Rat IgG2a DyLight 649 . Figure B. FITC conjugated Mouse anti Canine CD3 and purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a DyLight 649 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. FITC conjugated Mouse anti Canine CD3 and purified Rat IgG2a isotype control detected with Goat anti Rat IgG2a DyLight 649 . Figure B. FITC conjugated Mouse anti Canine CD3 and purified Rat anti Canine CD90 detected with Goat anti Rat IgG2a DyLight 649 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. A488 conjugated Rat anti Canine CD44 and RPE conjugated Rat IgG2a isotype control . Figure B. A488 conjugated Rat anti Canine CD44 and RPE conjugated Rat anti Canine CD45R . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. A488 conjugated Rat anti Canine CD44 and RPE conjugated Rat IgG2a isotype control . Figure B. A488 conjugated Rat anti Canine CD44 and RPE conjugated Rat anti Canine CD45R . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. RPE conjugated Rat anti Canine CD45R and A488 conjugated Rat IgG1 isotype control . Figure B. RPE conjugated Rat anti Canine CD45R and A488 conjugated Rat anti Canine CD44 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Testing Data
(Figure A. RPE conjugated Rat anti Canine CD45R and A488 conjugated Rat IgG1 isotype control . Figure B. RPE conjugated Rat anti Canine CD45R and A488 conjugated Rat anti Canine CD44 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)
Related Product Information for IgG2a negative control
Description: Rat IgG2a isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes trinitrophenol (TNP).
Summary: Rat IgG2a negative control, a rat monoclonal raised against trinitrophenol, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2a test antibodies to the surface of human and mouse cells in flow cytometry.
Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
Summary: Rat IgG2a negative control, a rat monoclonal raised against trinitrophenol, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2a test antibodies to the surface of human and mouse cells in flow cytometry.
Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
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Product Notes
The IgG2a (Catalog #AAA225675) is a Negative Control produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat IgG2a Negative Control reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IgG2a can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the IgG2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG2a, Negative Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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