Rabbit anti-Human Heat Shock Protein 27 Polyclonal Antibody | anti-HSPB1 antibody

Rabbit anti Heat Shock Protein 27 (pSer15)

Cat. Num. AAA225264

• Gene Names: HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified whole serum by affinity chromatography.
• Synonyms: Heat Shock Protein 27; Polyclonal Antibody; Rabbit anti Heat Shock Protein 27 (pSer15); Heat Shock Protein 27 (pSer15) Antibody; anti-HSPB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified whole serum by affinity chromatography.
• Form/Format: Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
• Sequence Length: 205

• Applicable Applications for anti-HSPB1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-Paraffin: 1:50-1:100; WB: 1:500-1:2000
• Immunogen: Phospho specific-peptide corresponding to residues surrounding serine 15 of human heat shock protein 27
• Preperation: Antiserum to heat shock protein 27 (pSer15) was raised by repeated immunization of rabbits with highly purified antigen.
• Preparation and Storage: Store at -20 degree C.; Storage in frost-free freezers is not recommended. This product should be stored undiluted.; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells untreated or treated with UV (for 15-30 minutes) using Rabbit anti heat shock protein 27 (pSer15) antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti heat shock protein 27 (pSer15) antibody)

Related Product Information for anti-HSPB1 antibody

Rabbit anti heat shock protein 27 (pSer15) antibody recognizes heat shock protein 27 when phosphorylated at serine 15. Heat shock protein 27 is a molecular chaperone protein. HSP27 exerts an anti-apoptotic effect via inhibition of the cytochrome-c/pro-caspase-9 pathway. Phosphorylation of HSP27 at serine 15 occurs via p38 MAPK. Phosphorylation of HSP27 at serine 15 contributes to significant alterations in the protein structure and protein-protein interactions of HSP27.

NCBI and Uniprot Product Information

• NCBI GI #: 19855073
• NCBI GeneID: 3315
• UniProt Accession #: P04792; Q6FI47; Q96C20; Q96EI7; Q9UC31; Q9UC34; Q9UC35; Q9UC36; B2R4N8
• Molecular Weight: 22,783 Da
• NCBI Official Full Name: Heat shock protein beta-1
• NCBI Official Synonym Full Names: heat shock protein family B (small) member 1
• NCBI Official Symbol: HSPB1
• NCBI Official Synonym Symbols: CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• NCBI Protein Information: heat shock protein beta-1
• UniProt Protein Name: Heat shock protein beta-1
• Protein Family: Heat shock protein
• UniProt Gene Name: HSPB1
• UniProt Synonym Gene Names: HSP27; HSP28; HspB1; HSP 27; SRP27

NCBI Description

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]

Uniprot Description

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).