Western Blot (WB)
(HSPBP1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HSPBP1 antibody detected the HSPBP1 protein (arrow).)
Rabbit anti-Human HSPBP1 Polyclonal Antibody | anti-HSPBP1 antibody
HSPBP1, NT (HSPBP1, HSPBP, Hsp70-binding protein 1, Heat shock protein-binding protein 1, Hsp70-binding protein 2, Hsp70-interacting protein 1, Hsp70-interacting protein 2)
Gene Names
HSPBP1; FES1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
HSPBP1; Polyclonal Antibody; NT (HSPBP1; HSPBP; Hsp70-binding protein 1; Heat shock protein-binding protein 1; Hsp70-binding protein 2; Hsp70-interacting protein 1; Hsp70-interacting protein 2); Anti -HSPBP1; anti-HSPBP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-HSPBP1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunogen
HSPBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the N-terminal region of human HSPBP1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(HSPBP1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HSPBP1 antibody detected the HSPBP1 protein (arrow).)
Western Blot (WB)
(HSPBP1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HSPBP1 antibody detected the HSPBP1 protein (arrow).)
Immunohistochemistry (IHC)
(HSPBP1 Antibody (N-term) (AP14772a)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HSPBP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(HSPBP1 Antibody (N-term) (AP14772a)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HSPBP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-HSPBP1 antibody
HSPBP1 inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.
Product Categories/Family for anti-HSPBP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,474 Da
NCBI Official Full Name
hsp70-binding protein 1
NCBI Official Synonym Full Names
HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1
NCBI Official Symbol
HSPBP1
NCBI Official Synonym Symbols
FES1
NCBI Protein Information
hsp70-binding protein 1; hspBP2; Hsp70 binding protein 1; hsp70-binding protein 2; hsp70 interacting protein; hsp70-interacting protein 1; hsp70-interacting protein 2; heat shock protein-binding protein 1
UniProt Protein Name
Hsp70-binding protein 1
Protein Family
UniProt Gene Name
HSPBP1
UniProt Synonym Gene Names
HSPBP; HspBP1; HspBP2
UniProt Entry Name
HPBP1_HUMAN
Uniprot Description
HSPBP1: Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 19q13.42
Molecular Function: enzyme inhibitor activity; protein binding
Biological Process: protein folding; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of catalytic activity
Research Articles on HSPBP1
Similar Products
Product Notes
The HSPBP1 hspbp1 (Catalog #AAA6001883) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSPBP1, NT (HSPBP1, HSPBP, Hsp70-binding protein 1, Heat shock protein-binding protein 1, Hsp70-binding protein 2, Hsp70-interacting protein 1, Hsp70-interacting protein 2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSPBP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the HSPBP1 hspbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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