Flow Cytometry (FC/FACS)
(Staining of lapine peripheral blood lymphocytes with Mouse anti Rabbit IgM (B cell marker):FITC)
Mouse anti-Rabbit IgM Monoclonal Antibody | anti-IgM antibody
MOUSE ANTI RABBIT IgM (B CELL MARKER): FITC
Reactivity
Rabbit
Applications
Flow Cytometry
Synonyms
IgM; Monoclonal Antibody; MOUSE ANTI RABBIT IgM (B CELL MARKER): FITC; anti-IgM antibody
Host
Mouse
Reactivity
Rabbit
Clonality
Monoclonal
Isotype
IgG1
Clone Number
NRBM
Specificity
IgM
Form/Format
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Sequence Length
112
Applicable Applications for anti-IgM antibody
Flow Cytometry (FC)
Application Notes
FC: Min: Neat/Max: 1/10
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers
0.09% Sodium Azide (NaN3), 1% Bovine Serum Albumin
Buffer Solution
Phosphate buffered saline
Max Ex/Em
Fluorophore : FITC
Excitation Max (nm): 490
Emission Max (nm): 525
Excitation Max (nm): 490
Emission Max (nm): 525
Fusion Partners
Spleen cells from immunised mice were fused with cells of the Mouse P3X63Ag8.653 myeloma cell line.
Preparation and Storage
Store at +4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Flow Cytometry (FC/FACS)
(Staining of lapine peripheral blood lymphocytes with Mouse anti Rabbit IgM (B cell marker):FITC)
Flow Cytometry (FC/FACS)
(Staining of lapine peripheral blood lymphocytes with Mouse anti Rabbit IgM (B cell marker):FITC)
Flow Cytometry (FC/FACS)
(Staining of lapine peripheral blood lymphocytes with Mouse anti Rabbit IgM (B cell marker):RPE )
Flow Cytometry (FC/FACS)
(Staining of lapine peripheral blood lymphocytes with Mouse anti Rabbit IgM (B cell marker):RPE )
Related Product Information for anti-IgM antibody
Mouse anti Rabbit IgM (B Cell Marker) antibody, clone NRBM specifically recognizes the μ chain of rabbit Ig.Mammalian IgM is produced and secreted by plasma cells located in bone marrow, lymph nodes and spleen. IgM is present in both a secreted polymeric form and as cell surface monomeric form on B cells. Clone NRBM has been used in number of studies to label IgM+ B cells (Dewals et al. 2011, Waclavicek et al. 2009) and as such can be considered a reliable marker of lagomorph (rabbit) B cells in flow cytometry.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The IgM (Catalog #AAA224828) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI RABBIT IgM (B CELL MARKER): FITC reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's IgM can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC). FC: Min: Neat/Max: 1/10 Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the IgM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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