Rat anti-Mouse CD64 Monoclonal Antibody | anti-CD64 antibody
RAT ANTI MOUSE CD64
Gene Names
Fcgr1; CD64; IGGHAFC; AI323638; AV092959; FcgammaRI
Reactivity
Mouse
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
CD64; Monoclonal Antibody; RAT ANTI MOUSE CD64; FcRI; Fcg1; anti-CD64 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
AT152-9
Specificity
CD64
Form/Format
Purified IgG-liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
404
Applicable Applications for anti-CD64 antibody
Immunohistochemistry (IHC) Formalin, Flow Cytometry (FC/FACS)
Application Notes
Immunohistochemistry- Frozen: 1/1000
Flow Cytometry: 1/10
Flow Cytometry Note: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul
Flow Cytometry: 1/10
Flow Cytometry Note: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Buffer Solution
TRIS buffered saline
Target Species
Mouse
Immunogen
HEK293F cells expressing CD64 and gamma chain
Fusion Partners
Spleen cells from immunised rats were fused with cells of the NS-1 myeloma cell line
Preparation and Storage
Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: Up to 12 months from date of despatch
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: Up to 12 months from date of despatch
Related Product Information for anti-CD64 antibody
Rat anti mouse CD64 antibody, clone AT152-9, recognizes mouse CD64 also known as FcRI. CD64 is a high affinity activatory receptor for IgG2a and a low affinity receptor for IgG2b and IgG3 type antibodies. The interaction between Fc receptors and antibodies play important roles in both the innate and adaptive immune responses.
CD64, through binding of the Fc segment of IgG, mediates phagocytosis and plays a role in antibody-dependent cellular cytotoxicity and clearance of immune complexes. In addition, CD64 also functions as an antigen capture for presentation to T-cells and also mediates the release of cytokines and reactive oxygen intermediates including interleukin (IL)-1, IL-6 and tumor necrosis factor (TNF) alpha. It is constitutively expressed on monocytes and macrophages, germinal centre dendritic cells and early myeloid lineage cells, but not lymphocytes. Expression on monocytes can be strongly upregulated by treatment with interferon (IFN) gamma or G-CSF, and can be induced on neutrophils and eosinophils by IFN gamma.
CD64, through binding of the Fc segment of IgG, mediates phagocytosis and plays a role in antibody-dependent cellular cytotoxicity and clearance of immune complexes. In addition, CD64 also functions as an antigen capture for presentation to T-cells and also mediates the release of cytokines and reactive oxygen intermediates including interleukin (IL)-1, IL-6 and tumor necrosis factor (TNF) alpha. It is constitutively expressed on monocytes and macrophages, germinal centre dendritic cells and early myeloid lineage cells, but not lymphocytes. Expression on monocytes can be strongly upregulated by treatment with interferon (IFN) gamma or G-CSF, and can be induced on neutrophils and eosinophils by IFN gamma.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
High affinity immunoglobulin gamma Fc receptor I
NCBI Official Synonym Full Names
Fc receptor, IgG, high affinity I
NCBI Official Symbol
Fcgr1
NCBI Official Synonym Symbols
CD64; IGGHAFC; AI323638; AV092959; FcgammaRI
NCBI Protein Information
high affinity immunoglobulin gamma Fc receptor I
UniProt Protein Name
High affinity immunoglobulin gamma Fc receptor I
UniProt Gene Name
Fcgr1
UniProt Synonym Gene Names
Fcg1; IgG Fc receptor I; FcRI
UniProt Entry Name
FCGR1_MOUSE
Uniprot Description
FCGR1A: High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Belongs to the immunoglobulin superfamily. FCGR1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Cellular Component: external side of plasma membrane; integral to membrane; lipid raft; membrane; plasma membrane
Molecular Function: IgG binding; IgG receptor activity; leukotriene receptor binding
Biological Process: antibody-dependent cellular cytotoxicity; antigen processing and presentation of exogenous antigen; antigen processing and presentation of exogenous peptide antigen via MHC class I; cell surface receptor linked signal transduction; defense response to bacterium; immune system process; innate immune response; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of phagocytosis; positive regulation of phagocytosis, engulfment; positive regulation of type IIa hypersensitivity; positive regulation of type III hypersensitivity; receptor-mediated endocytosis; regulation of immune response; response to bacterium
Research Articles on CD64
Similar Products
Product Notes
The CD64 fcgr1 (Catalog #AAA224780) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The RAT ANTI MOUSE CD64 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD64 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin, Flow Cytometry (FC/FACS). Immunohistochemistry- Frozen: 1/1000 Flow Cytometry: 1/10 Flow Cytometry Note: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul. Researchers should empirically determine the suitability of the CD64 fcgr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD64, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
