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Western Blot (WB) (Anti-FCGR1 rabbit polyclonal antibody at 1:500 dilutionLane A: RAW264.7Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:43 kDaObserved band size:40 kDa(We are unsure as to the identity of these extra bands.))

Rabbit anti-Mouse CD64 Polyclonal Antibody | anti-FCGR1 antibody

Anti-CD64 Antibody, Rabbit Polyclonal

Gene Names
Fcgr1; CD64; IGGHAFC; AI323638; AV092959; FcgammaRI
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
CD64; Polyclonal Antibody; Anti-CD64 Antibody; Rabbit Polyclonal; CD64/FCGR1 Antibody; Rabbit PAb; Antigen Affinity Purified; Fc receptor; IgG; high affinity I; Anti-AI323638 Antibody; Anti-AV092959 Antibody; Anti-FcgammaRI Antibody; Anti-IGGHAFC Antibody; anti-FCGR1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse FCGR1/CD64
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-FCGR1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:5000-1:10,000
Immunogen
Recombinant Mouse FCGR1 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse FCGR1 extracellular domain (rM FCGR1). FCGR1 specific IgG was purified by mouse FCGR1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-FCGR1 rabbit polyclonal antibody at 1:500 dilutionLane A: RAW264.7Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:43 kDaObserved band size:40 kDa(We are unsure as to the identity of these extra bands.))

Western Blot (WB) (Anti-FCGR1 rabbit polyclonal antibody at 1:500 dilutionLane A: RAW264.7Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:43 kDaObserved band size:40 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-FCGR1 antibody
High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNgammaandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.
Product Categories/Family for anti-FCGR1 antibody
References
Allen J.M., et al., (1988), Nucleotide sequence of three cDNAs for the human high affinity Fc receptor (FcRI). Nucleic Acids Res. 16:11824-11824.Allen J.M., et al., (1989), Isolation and expression of functional high-affinity Fc receptor complementary DNAs.Science 243:378-381.Porges A.J., et al., (1992), Novel Fc gamma receptor I family gene products in human mononuclear cells.J. Clin. Invest. 90:2102-2109.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,888 Da
NCBI Official Full Name
high affinity immunoglobulin gamma Fc receptor I
NCBI Official Synonym Full Names
Fc receptor, IgG, high affinity I
NCBI Official Symbol
Fcgr1
NCBI Official Synonym Symbols
CD64; IGGHAFC; AI323638; AV092959; FcgammaRI
NCBI Protein Information
high affinity immunoglobulin gamma Fc receptor I
UniProt Protein Name
High affinity immunoglobulin gamma Fc receptor I
UniProt Gene Name
Fcgr1
UniProt Synonym Gene Names
Fcg1; IgG Fc receptor I; FcRI
UniProt Entry Name
FCGR1_MOUSE

Uniprot Description

FCGR1A: High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Belongs to the immunoglobulin superfamily. FCGR1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: membrane; integral to membrane; plasma membrane; lipid raft; external side of plasma membrane

Molecular Function: leukotriene receptor binding; IgG binding; IgG receptor activity

Biological Process: receptor-mediated endocytosis; regulation of immune response; immune system process; positive regulation of type III hypersensitivity; phagocytosis, recognition; antigen processing and presentation of exogenous antigen; antibody-dependent cellular cytotoxicity; cell surface receptor linked signal transduction; response to bacterium; positive regulation of type IIa hypersensitivity; positive regulation of phagocytosis; defense response to bacterium; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; positive regulation of phagocytosis, engulfment; phagocytosis, engulfment

Research Articles on FCGR1

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Product Notes

The FCGR1 fcgr1 (Catalog #AAA8110946) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD64 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD64 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:5000-1:10,000. Researchers should empirically determine the suitability of the FCGR1 fcgr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD64, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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