Rat CD9 Monoclonal Antibody | anti-CD9 antibody

RAT ANTI MOUSE CD9

Cat. Num. AAA224489

• Gene Names: Cd9; Tspan29
• Applications: Flow Cytometry, Immunoprecipitation, Western Blot
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
• Synonyms: CD9; Monoclonal Antibody; RAT ANTI MOUSE CD9; anti-CD9 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: MF1
• Specificity: CD9
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 226

• Applicable Applications for anti-CD9 antibody: Flow cytometry (FC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.; Western Blot: This item detects a band of approximately 25kDa in cell lysates.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Mouse
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt.; When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Testing Data #1

(Staining of Mouse Peripheral Platelets with Rat anti Mouse CD9:Endotoxin Low (MBS224726))

Testing Data #2

(Staining of Mouse Peripheral Platelets with Rat anti Mouse CD9 (MBS224489))

Testing Data #3

(Staining of Mouse Peripheral Platelets with Rat anti Mouse CD9 (MBS224547))

Testing Data #4

(Staining of Mouse Peripheral Platelets with Rat anti Mouse CD9:RPE(MBS224632))

Related Product Information for anti-CD9 antibody

Rat anti Mouse CD9 antibody, clone MF1 recognizes mouse CD9, a 25kD member of the tetraspanin family of transmembrane proteins. The tetraspanin family are multifunctional molecules which bind to a wide range of transmembrane proteins and intracellular signalling molecules. Tetraspanins facilitate the formation of molecular complexes which play a role in cell activation, proliferation, motility, fusion and apoptosis. CD9 is ubiquitously expressed, with high levels found on platelets and in male reproductive tissues. In mouse haematopoietic tissues, CD9 is expressed by myeloid and stromal cells. CD9 is known to complex with integrins and other transmembrane proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation.

NCBI and Uniprot Product Information

• NCBI GI #: 6680894
• NCBI GeneID: 12527
• NCBI Accession #: NP_031683.1
• NCBI GenBank Nucleotide #: NM_007657.3
• UniProt Accession #: P40240; Q3U9W0
• NCBI Official Full Name: CD9 antigen
• NCBI Official Synonym Full Names: CD9 antigen
• NCBI Official Symbol: Cd9
• NCBI Official Synonym Symbols: Tspan29
• NCBI Protein Information: CD9 antigen
• UniProt Protein Name: CD9 antigen
• Protein Family: CD9 antigen
• UniProt Gene Name: Cd9
• UniProt Entry Name: CD9_MOUSE

Uniprot Description

CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Cell adhesion

Cellular Component: extracellular space; focal adhesion; cell surface; membrane; apical plasma membrane; integral to membrane; plasma membrane; vesicle; external side of plasma membrane

Molecular Function: integrin binding; protein binding

Biological Process: negative regulation of cell proliferation; fusion of sperm to egg plasma membrane; single fertilization; cell motility; cell adhesion; paranodal junction assembly