Rabbit anti-Monkey ATG1 Polyclonal Antibody | anti-ATG1 antibody

RABBIT ANTI ATG1

Cat. Num. AAA224333

• Gene Names: ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
• Reactivity: Monkey
• Applications: Immunohistochemistry, Western Blot
• Synonyms: ATG1; Polyclonal Antibody; RABBIT ANTI ATG1; anti-ATG1 antibody

• Host: Rabbit
• Reactivity: Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises Human ULK1, a ubiquitously expressed cytoplasmic protein belonging to the serine/threonine protein kinase family. ; ; ULK1 is an autophagy related protein which is required for the formation of autophagosomes. ULK1 is the the target of the TOR kinase signalling pathway regulating autophagy and forms a complex with ATG13 and RB1CC1. ; ; ULK1 is strongly expressed in a wide range of adult tissues including heart, lung, kidney, brain, liver, pancreatic tissue and skeletal muscle.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: Total protein concentration 0.5mg/ml (varies by lot)
• Sequence Length: 1,050

• Applicable Applications for anti-ATG1 antibody: Immunohistology Paraffin, Western Blot (WB)
• Application Notes: Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. ; Immunohistology - Paraffin: Minimum Dilution: 10ug/ml; Western Blotting: Minimum Dilution: 1; Maximum Dilution: 3ug/ml
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3); 0.2% Gelatin
• Immunogen: A synthetic peptide corresponding to an amino acid sequence within the region 320-370 of Human ULK1.; Histology Positive Control Tissue
• Antiserum Preparation: Antiserum to human ULK1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human kidney with Rabbit anti Human ULK1 (MBS224333))

Related Product Information for anti-ATG1 antibody

MBS224333 recognises Human ULK1, a ubiquitously expressed cytoplasmic protein belonging to the serine/threonine protein kinase family. ULK1 is an autophagy related protein which is required for the formation of autophagosomes. ULK1 is the the target of the TOR kinase signalling pathway regulating autophagy and forms a complex with ATG13 and RB1CC1. ULK1 is strongly expressed in a wide range of adult tissues including heart, lung, kidney, brain, liver, pancreatic tissue and skeletal muscle.

NCBI and Uniprot Product Information

• NCBI GI #: 4507831
• NCBI GeneID: 8408
• NCBI Accession #: NP_003556.1
• NCBI GenBank Nucleotide #: NM_003565.2
• UniProt Accession #: O75385; Q9UQ28
• Molecular Weight: 112,631 Da
• NCBI Official Full Name: serine/threonine-protein kinase ULK1
• NCBI Official Synonym Full Names: unc-51 like autophagy activating kinase 1
• NCBI Official Symbol: ULK1
• NCBI Official Synonym Symbols: ATG1; ATG1A; UNC51; hATG1; Unc51.1
• NCBI Protein Information: serine/threonine-protein kinase ULK1; ATG1 autophagy related 1 homolog; autophagy-related protein 1 homolog
• UniProt Protein Name: Serine/threonine-protein kinase ULK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: ULK1
• UniProt Synonym Gene Names: KIAA0722; ATG1; hATG1
• UniProt Entry Name: ULK1_HUMAN

Uniprot Description

ULK1: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. Interacts with GABARAP and GABARAPL2. Interacts (via C- terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation. Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

Protein type: Protein kinase, Other; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Autophagy; Kinase, protein; Other group; ULK family

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: neuron projection; cytoplasmic vesicle membrane; cell soma; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein complex binding; protein kinase binding; Rab GTPase binding; ATP binding

Biological Process: axon extension; protein amino acid autophosphorylation; neurite regeneration; negative regulation of collateral sprouting; cerebellar granule cell differentiation; protein amino acid phosphorylation; radial glia guided migration of granule cell; response to starvation; protein localization; cellular response to nutrient levels; receptor internalization; Ras protein signal transduction; regulation of autophagy; regulation of nerve growth factor receptor signaling pathway; positive regulation of macroautophagy; neurite development; positive regulation of autophagy; autophagic vacuole formation