Rabbit anti-Human CCK4 Polyclonal Antibody | anti-CCK4 antibody

CCK4 (PTK7), NT (PTK7, CCK4, Inactive tyrosine-protein kinase 7, Colon carcinoma kinase 4, Protein-tyrosine kinase 7, Pseudo tyrosine kinase receptor 7, Tyrosine-protein kinase-like 7) (MaxLight 405)

Cat. Num. AAA6281997

• Gene Names: PTK7; CCK4; CCK-4
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: CCK4; Polyclonal Antibody; CCK4 (PTK7); NT (PTK7; Inactive tyrosine-protein kinase 7; Colon carcinoma kinase 4; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; Tyrosine-protein kinase-like 7) (MaxLight 405); PTK7; Tyrosine-protein kinase-like 7; anti-CCK4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CCK4.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-CCK4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 21-52 in the N-terminal region of human CCK4, UniProt Accession #Q13308.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell lysates (2ug/lane) using 033359 either nontransfected (Lane 1) or transiently transfected with the PTK7 gene (Lane 2).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted with 033359, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of 033359 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H460 cells using 033359 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CCK4 antibody

CCK4 may function as a cell adhesion molecule. Although it belongs to the insuline receptor subfamily of the Tyr protein kinases, it likely lacks the catalytic activity of a tyrosine kinase. It may be connected to the pathophysiology of colon carcinomas and/or may represent a tumor progression marker. This Type I membrane protein is highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes, but weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle, and not in colon. It is also expressed in erythroleukemia cells.

Product Categories/Family for anti-CCK4 antibody

Antibodies; Protein Kinases

References

1. Zhang, H., et al., Nat. Biotechnol. 21(6):660-666 (2003). 2. Park, S.K., et al., J. Biochem. 119(2):235-239 (1996). 3. Mossie, K., et al., Oncogene 11(10):2179-2184 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 47938093
• NCBI GeneID: 5754
• UniProt Accession #: Q13308; Q13417; Q5T650; Q6IQ54; Q8NFA5; Q8NFA6; Q8NFA7; Q8NFA8; A8K974; B7Z477; E9PFZ5
• Molecular Weight: 119,197 Da
• NCBI Official Full Name: PTK7 protein tyrosine kinase 7
• NCBI Official Synonym Full Names: protein tyrosine kinase 7 (inactive)
• NCBI Official Symbol: PTK7
• NCBI Official Synonym Symbols: CCK4; CCK-4
• NCBI Protein Information: inactive tyrosine-protein kinase 7; colon carcinoma kinase 4; PTK7 protein tyrosine kinase 7; tyrosine-protein kinase-like 7; pseudo tyrosine kinase receptor 7
• UniProt Protein Name: Inactive tyrosine-protein kinase 7
• UniProt Gene Name: PTK7
• UniProt Synonym Gene Names: CCK4; CCK-4
• UniProt Entry Name: PTK7_HUMAN

NCBI Description

This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

CCK4: Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; CCK4 family

Chromosomal Location of Human Ortholog: 6p21.1-p12.2

Cellular Component: focal adhesion; integral to plasma membrane; intercellular junction

Molecular Function: protein binding; protein-tyrosine kinase activity; ATP binding

Biological Process: cell migration; peptidyl-tyrosine phosphorylation; wound healing; convergent extension; actin cytoskeleton reorganization; neural tube closure; establishment of epithelial cell polarity; establishment of planar polarity; Wnt receptor signaling pathway through beta-catenin; cell adhesion; signal transduction