Rabbit ULK2 Polyclonal Antibody | anti-ULK2 antibody

RABBIT ANTI MOUSE ULK2

Cat. Num. AAA224302

• Gene Names: Ulk2; Unc51.2; AU015340; mKIAA0623; A830085I22Rik
• Reactivity: Human (Expected from Sequence), Rat (Expected from Sequence)
• Applications: Western Blot
• Synonyms: ULK2; Polyclonal Antibody; RABBIT ANTI MOUSE ULK2; anti-ULK2 antibody

• Host: Rabbit
• Reactivity: Human (Expected from Sequence), Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises mouse Ulk2 (Serine/threonine-protein kinase Ulk2), otherwise known as Unc-51-like kinase 2, a widely expressed mammalian Atg1 homologue and member of the protein kinase superfamily, which shares structural similarity with a serine/threonine kinase found in C. elegans, known to be involved in axonal elongation. ; Studies have shown that murine Ulk2 and closely-related Ulk1, localize to autophagic isolation membranes under starvation conditions, and that the binding of Atg13 (Autophagy-related protein 13) to Ulk1 and Ulk2, stabilizes and activates them, and facilitates the phosphorylation of FIP200, a multifunctional protein involved in cell migration, proliferation, apoptosis, and tumor suppression.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 1,037

• Applicable Applications for anti-ULK2 antibody: Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 130kDa in HEK-293T cell lysates transfected with mouse ULK2.; Western Blotting: Minimum Dilution: 2.5; Maximum Dilution: 5.0ug/ml
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: KLH conjugated synthetic peptide corresponding to amino acids 370-382 of mouse Ulk2. The corresponding sequence differs by two amino acids in rat and three amino acids in human.; Buffer Solution
• Antiserum Preparation: Antiserum to mouse Ulk2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-ULK2 antibody

MBS224302 specifically recognises mouse Ulk2 (Serine/threonine-protein kinase Ulk2), otherwise known as Unc-51-like kinase 2, a widely expressed mammalian Atg1 homologue and member of the protein kinase superfamily, which shares structural similarity with a serine/threonine kinase found in C. elegans, known to be involved in axonal elongation.Studies have shown that murine Ulk2 and closely-related Ulk1, localize to autophagic isolation membranes under starvation conditions, and that the binding of Atg13 (Autophagy-related protein 13) to Ulk1 and Ulk2, stabilizes and activates them, and facilitates the phosphorylation of FIP200, a multifunctional protein involved in cell migration, proliferation, apoptosis, and tumor suppression.

NCBI and Uniprot Product Information

• NCBI GI #: 238231390
• NCBI GeneID: 29869
• NCBI Accession #: NP_038909.3
• NCBI GenBank Nucleotide #: NM_013881.4
• UniProt Accession #: Q9QY01; Q80TV7; Q9WTP4
• Molecular Weight: 112,877 Da
• NCBI Official Full Name: serine/threonine-protein kinase ULK2
• NCBI Official Synonym Full Names: unc-51 like kinase 2
• NCBI Official Symbol: Ulk2
• NCBI Official Synonym Symbols: Unc51.2; AU015340; mKIAA0623; A830085I22Rik
• NCBI Protein Information: serine/threonine-protein kinase ULK2; serine/threonine-protein kinase Unc51.2; unc-51-like kinase 2
• UniProt Protein Name: Serine/threonine-protein kinase ULK2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Ulk2
• UniProt Synonym Gene Names: Kiaa0623
• UniProt Entry Name: ULK2_MOUSE

Uniprot Description

ULK2: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

Protein type: EC 2.7.11.1; Kinase, protein; Autophagy; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Other group; ULK family

Cellular Component: membrane; pre-autophagosomal structure membrane; cytoplasmic vesicle; cytosol

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: response to starvation; nervous system development; axonogenesis; axon extension; protein amino acid autophosphorylation; autophagy; regulation of autophagy; negative regulation of collateral sprouting; signal transduction; phosphorylation; protein amino acid phosphorylation; autophagic vacuole formation