Sheep anti-Mouse ERYTHROPOIETIN RECEPTOR Polyclonal Antibody | anti-EPOR antibody

SHEEP ANTI HUMAN ERYTHROPOIETIN RECEPTOR

Cat. Num. AAA224081

• Gene Names: EPOR; EPO-R
• Reactivity: Mouse
• Applications: ELISA, Immunoprecipitation, Western Blot
• Synonyms: ERYTHROPOIETIN RECEPTOR; Polyclonal Antibody; SHEEP ANTI HUMAN ERYTHROPOIETIN RECEPTOR; anti-EPOR antibody

• Host: Sheep
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises Erythropoietin Receptor (EPOR), a type I transmembrane protein and member of the type I cytokine receptor family, expressed by erythroid cells and erythroid progenitor cells, which acts as a receptor for erythropoietin (EPO) to mediate erythroblast proliferation and differentiation, and maintain progenitor survival. Binding of EPO to EPOR, results in the dimerization of the EPOR and the triggering of the JAK2/STAT5 signaling cascade, and in some cell types activation of STAT1 and STAT3. Several isoforms of EPOR exist: EPOR-F, EPOR-S and EPOR-T, and a functional EPOR is found on endothelial cells and cardiomyocytes within the cardiovascular system, suggesting a role in cardiac function. This item has been shown to block Iodine125 labelled erythropoietin ligand binding using erythroleukemia cell line TF-1 with 3000 EPOR/cell in neutralization studies, see Harris, K.W.et al. for details.This item is reported as suitable for use in immunocytochemistry.This item has been reported for use in functional studies. Removal of Sodium Azide is recommended prior to use in functional assays. The recommended concentration for functional assays is 500ug/ml.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 508

• Applicable Applications for anti-EPOR antibody: ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: This product is suitable for use in indirect ELISA applications.; Western Blot: This item detects a 49kDa band of EPOR-GST on SDS-PAGE.; ELISA: Minimum Dilution: 50ng/ml; Immunoprecipitation: Minimum Dilution: 10ug/ml; Western Blotting: Minimum Dilution: 10ug/ml
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: Partial cDNA of the extracellular domain of human EPOR expressed as a GST fusion protein in E Coli. ; Buffer Solution
• Antiserum Preparation: Antiserum to human EPOR was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-EPOR antibody

MBS224081 recognises Erythropoietin Receptor (EPOR), a type I transmembrane protein and member of the type I cytokine receptor family, expressed by erythroid cells and erythroid progenitor cells, which acts as a receptor for erythropoietin (EPO) to mediate erythroblast proliferation and differentiation, and maintain progenitor survival. Binding of EPO to EPOR, results in the dimerization of the EPOR and the triggering of the JAK2/STAT5 signaling cascade, and in some cell types activation of STAT1 and STAT3. Several isoforms of EPOR exist: EPOR-F, EPOR-S and EPOR-T, and a functional EPOR is found on endothelial cells and cardiomyocytes within the cardiovascular system, suggesting a role in cardiac function. MBS224081 has been shown to block Iodine125 labelled erythropoietin ligand binding using erythroleukemia cell line TF-1 with 3000 EPOR/cell in neutralization studies, see Harris, K.W.et al. for details. MBS224081 is reported as suitable for use in immunocytochemistry. MBS224081 has been reported for use in functional studies. Removal of Sodium Azide is recommended prior to use in functional assays. MyBioSource recommend the use of EQU003 for this purpose. The recommended concentration for functional assays is 500ug/ml.

NCBI and Uniprot Product Information

• NCBI GI #: 4503591
• NCBI GeneID: 2057
• NCBI Accession #: NP_000112.1
• NCBI GenBank Nucleotide #: NM_000121.3
• Molecular Weight: 35,809 Da
• NCBI Official Full Name: erythropoietin receptor
• NCBI Official Synonym Full Names: erythropoietin receptor
• NCBI Official Symbol: EPOR
• NCBI Official Synonym Symbols: EPO-R
• NCBI Protein Information: erythropoietin receptor
• UniProt Protein Name: Erythropoietin receptor
• Protein Family: Erythropoietin receptor
• UniProt Gene Name: EPOR
• UniProt Synonym Gene Names: EPO-R
• UniProt Entry Name: EPOR_HUMAN

NCBI Description

This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane; extracellular region

Molecular Function: identical protein binding; protein binding; erythropoietin receptor activity

Biological Process: heart development; brain development; decidualization; signal transduction

Disease: Erythrocytosis, Familial, 1