Typical Testing Data/Standard Curve (for reference only)
Goat Erythropoietin Receptor ELISA Kit | EPOR elisa kit
Goat Erythropoietin Receptor ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This EPOR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat EPOR. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY EPOR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EPOR antibody and an EPOR-HRP conjugate. The assay sample and buffer are incubated together with EPOR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensi ty of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPOR concentration since EPOR from samples and EPOR-HRP conjugate compete for the anti-EPOR antibody binding site. Since the number of sites is limited, as more sites are occupied by EPOR from the sample, fewer sites are left to bind EPOR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPOR concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
Uniprot Description
EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 19p13.3-p13.2
Cellular Component: integral to plasma membrane; extracellular region
Molecular Function: identical protein binding; protein binding; erythropoietin receptor activity
Biological Process: heart development; brain development; decidualization; signal transduction
Disease: Erythrocytosis, Familial, 1
Research Articles on EPOR
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Product Notes
The Goat EPOR epor (Catalog #AAA740136) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA740136 ELISA Kit recognizes Goat EPOR. It is sometimes possible for the material contained within the vial of "Erythropoietin Receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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