Goat EZRIN Polyclonal Antibody | anti-EZRIN antibody

GOAT ANTI HUMAN EZRIN

Cat. Num. AAA222872

• Gene Names: EZR; CVL; CVIL; VIL2; HEL-S-105
• Reactivity: Dog (Expected from Sequence)
• Applications: ELISA, Western Blot
• Synonyms: EZRIN; Polyclonal Antibody; GOAT ANTI HUMAN EZRIN; anti-EZRIN antibody

• Host: Goat
• Reactivity: Dog (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 586

• Applicable Applications for anti-EZRIN antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 80kDa in human placenta and duodenum cell lysates.; ELISA: Maximum Dilution: 1/16,000; Western Blotting: Minimum Dilution: 1.0; Maximum Dilution: 3.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin
• Buffer Solution: TRIS buffered saline
• Antiserum Preparation: Antiserum to human ezrin was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-EZRIN antibody

Goat anti Human Ezrin antibody recognizes human ezrin, otherwise known as p81 or villin 2, Ezrin is a 586 amino acid ~80kDa cytoplasmic peripheral membrane protein containing a single FERM domain and member of the ezrin, radixin, moesin (ERM) protein family, which acts as protein-tyrosine kinase substrate. Ezrin is enriched in microvilli and other cell surface structures and plays a key role in cell surface structure adhesion, membrane trafficking, motility and organization (Ng et al. 2001). Ezrin acts as a linker protein between the plasma membrane and the actin cytoskeleton, and provides anchorage for cell surface molecules such as ICAM and CD44 (Finnerty et al. 2004). Studies have implicated ezrin in the metastatic capacity of various types of human tumours including breast, prostate, bone, endometrial and colon cancers (Ohtani et al. 1999, Elzaqheid et al. 2008).

NCBI and Uniprot Product Information

• NCBI GI #: 161702986
• NCBI GeneID: 7430
• NCBI Accession #: NP_001104547.1
• NCBI GenBank Nucleotide #: NM_001111077.1
• UniProt Accession #: P15311; P23714; Q4VX75; Q96CU8; Q9NSJ4; E1P5A8
• Molecular Weight: 69,413 Da
• NCBI Official Full Name: ezrin
• NCBI Official Synonym Full Names: ezrin
• NCBI Official Symbol: EZR
• NCBI Official Synonym Symbols: CVL; CVIL; VIL2; HEL-S-105
• NCBI Protein Information: ezrin; cytovillin 2; epididymis secretory protein Li 105; p81; villin 2 (ezrin); villin-2
• UniProt Protein Name: Ezrin
• Protein Family: Ezrin
• UniProt Gene Name: EZR
• UniProt Synonym Gene Names: VIL2
• UniProt Entry Name: EZRI_HUMAN

NCBI Description

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Ezrin: a member of the ERM family which includes radixin and moesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Plays a key role in cell surface structure adhesion, migration, and organization.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: cortical cytoskeleton; extracellular space; focal adhesion; microvillus; basolateral plasma membrane; T-tubule; actin filament; cytosol; lipid raft; actin cytoskeleton; ruffle; extrinsic to membrane; membrane; microvillus membrane; apical part of cell; apical plasma membrane; plasma membrane; nucleolus; uropod; vesicle; filopodium

Molecular Function: actin filament binding; protein domain specific binding; protein binding; cell adhesion molecule binding

Biological Process: actin filament bundle formation; axon guidance; microvillus biogenesis; regulation of cell shape; leukocyte adhesion; filopodium formation; cytoskeletal anchoring; receptor internalization; membrane to membrane docking; establishment of epithelial cell polarity