Rabbit TRPA1 Polyclonal Antibody | anti-TRPA1 antibody

RABBIT ANTI HUMAN TRPA1

Cat. Num. AAA222811

• Gene Names: TRPA1; FEPS; ANKTM1
• Applications: Immunohistochemistry
• Synonyms: TRPA1; Polyclonal Antibody; RABBIT ANTI HUMAN TRPA1; anti-TRPA1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects Transient receptor potential A1 (TRPA1), an excitatory cation channel that is highly expressed in nociceptive neurons and plays an important role in inflammatory pain. TRPA1 mediates the response to the proalgesic bradykinin as well as to pungent irritants found in mustards, garlic, and cinnamon. ; ; TRPA1 may also be involved in hearing (as the mammalian hair cell mechanotransducer) and in the detection of cold stimuli.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 1119

• Applicable Applications for anti-TRPA1 antibody: Immunohistology Paraffin*
• Application Notes: Immunohistology - Paraffin: Minimum Dilution: 10; Maximum Dilution: 20ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Perservative Stabilisers: 0.1% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide: internal domain of human TRPA1, conjugated to Keyhole Limpet Hemocyanin (KLH).; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to human TRPA1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of human spinal cord doesal root gangion with Rabbit anti Human TRPA1 (MBS222811))

Related Product Information for anti-TRPA1 antibody

MBS222811 detects Transient receptor potential A1 (TRPA1), an excitatory cation channel that is highly expressed in nociceptive neurons and plays an important role in inflammatory pain. TRPA1 mediates the response to the proalgesic bradykinin as well as to pungent irritants found in mustards, garlic, and cinnamon. TRPA1 may also be involved in hearing (as the mammalian hair cell mechanotransducer) and in the detection of cold stimuli.

NCBI and Uniprot Product Information

• NCBI GI #: 116534990
• NCBI GeneID: 8989
• NCBI Accession #: NP_015628.2
• NCBI GenBank Nucleotide #: NM_007332.2
• UniProt Accession #: O75762; A6NIN6
• Molecular Weight: 127,501 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily A member 1
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily A, member 1
• NCBI Official Symbol: TRPA1
• NCBI Official Synonym Symbols: FEPS; ANKTM1
• NCBI Protein Information: transient receptor potential cation channel subfamily A member 1; ankyrin-like with transmembrane domains 1; transformation-sensitive protein p120
• UniProt Protein Name: Transient receptor potential cation channel subfamily A member 1
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPA1
• UniProt Synonym Gene Names: ANKTM1
• UniProt Entry Name: TRPA1_HUMAN

NCBI Description

The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control. [provided by RefSeq, Jul 2008]

Uniprot Description

TRPA1: Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)- tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system. Belongs to the transient receptor (TC 1.A.4) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: stereocilium bundle; integral to plasma membrane; plasma membrane

Molecular Function: channel activity; calcium channel activity

Biological Process: response to drug; detection of mechanical stimulus involved in sensory perception of pain; detection of chemical stimulus involved in sensory perception of pain; response to hydrogen peroxide; response to pain; ion transport; thermoception; response to cold; transmembrane transport

Disease: Episodic Pain Syndrome, Familial, 1