Rabbit GAP43 Polyclonal Antibody | anti-GAP43 antibody

RABBIT ANTI GAP43 (pSer41)

Cat. Num. AAA221792

• Gene Names: GAP43; B-50; PP46
• Reactivity: Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Monkey (Expected from Sequence), Mouse (Expected from Sequence), Xenopus (Expected from Sequence), Zebrafish (Expected from Se
• Applications: Western Blot
• Synonyms: GAP43; Polyclonal Antibody; RABBIT ANTI GAP43 (pSer41); anti-GAP43 antibody

• Host: Rabbit
• Reactivity: Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Monkey (Expected from Sequence), Mouse (Expected from Sequence), Xenopus (Expected from Sequence), Zebrafish (Expected from Se
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item is specific for Growth Associated Peptide 43 (GAP-43), when phosphorylated at serine 41. ; ; GAP-43 is expressed in neuronal growth cones during development and during axonal regeneration and is phosphorylated at Ser41 by protein kinase C.
• Form/Format: Purified; Purified IgG - liquid
• Sequence Length: 274

• Applicable Applications for anti-GAP43 antibody: Western Blot (WB)*
• Application Notes: Western Blot: This item detects a band of approximately 50kDa in rat cortex lysates.; Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
• Perservative Stabilisers: 0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol
• Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within GAP-43 which includes phosphorylated Ser41.; Buffer Solution
• Antiserum Preparation: Antisera to phosphorylated GAP-43 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of despatch.

Related Product Information for anti-GAP43 antibody

MBS221792 is specific for Growth Associated Peptide 43 (GAP-43), when phosphorylated at serine 41. GAP-43 is expressed in neuronal growth cones during development and during axonal regeneration and is phosphorylated at Ser41 by protein kinase C.

NCBI and Uniprot Product Information

• NCBI GI #: 194248057
• NCBI GeneID: 2596
• NCBI Accession #: NP_001123536.1
• NCBI GenBank Nucleotide #: NM_001130064.1
• UniProt Accession #: P17677; A8K0Y4
• Molecular Weight: 28,766 Da
• NCBI Official Full Name: neuromodulin isoform 1
• NCBI Official Synonym Full Names: growth associated protein 43
• NCBI Official Symbol: GAP43
• NCBI Official Synonym Symbols: B-50; PP46
• NCBI Protein Information: neuromodulin; axonal membrane protein GAP-43; calmodulin-binding protein P-57; growth-associated protein 43; nerve growth-related peptide GAP43; neural phosphoprotein B-50; neuron growth-associated protein 43; protein F1
• UniProt Protein Name: Neuromodulin
• Protein Family: Neuromodulin
• UniProt Gene Name: GAP43
• UniProt Entry Name: NEUM_HUMAN

NCBI Description

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GAP43: This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Belongs to the neuromodulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: filopodium membrane; axon; cytoplasm; postsynaptic density; plasma membrane; cell junction

Molecular Function: calmodulin binding; protein binding

Biological Process: cell fate commitment; glial cell differentiation; tissue regeneration; regulation of growth; protein kinase C activation; axon choice point recognition; response to wounding; regulation of filopodium formation